Human T Cell Kit

N° du produit ABIN1379761

Aperçu rapide pour Human T Cell Kit (ABIN1379761)

Application: Flow Cytometry (FACS), Intracellular Staining (ICS)

Détail du produit

Marque: BD Phosflow™

Attributs du produit: The BD Phosflow™ T Cell Kit Lyophilized Cells contains five vials each of lyophilized Treated and Untreated Human Control Cells. Each vial of control cells contains fixed, permeabilized, and lyophilized human peripheral blood leukocytes (PBL). The cells are intended to serve as biological controls for antibody staining in BD Phosflow studies designed to analyze cell signaling events associated with T-cell activation. These events include the phosphorylation status of signaling molecules that mediate the intracellular MAPK and JAK/STAT signaling pathway responses transduced by stimulated human CD4+ and CD8+ T cells.
The BD Phosflow™ Treated Human Control Cells - T cells are PBL that were stimulated to express easily detectable intracellular levels of several phosphorylated signaling molecules including the serine/threonine kinases, Erk1/2 (p44/p42 MAPK) and p38 MAPK and Signal Transducers and Activators of Transcription (STAT) proteins, Stat1, Stat3, Stat5, and Stat6 as determined by multicolor BD Phosflow™ analysis. The treated control cells were activated with phorbol 12-myristate 13-acetate (PMA), interferon-alpha (IFN-α), interleukin-2 (IL-2), IL-4, and IL-6. These activators are known to induce the cellular phosphorylation of the specified molecules that are often targeted in T cell signaling studies. Each vial of BD Phosflow™ Untreated Human Control Cells - T cells contains PBL from the same donor as the treated cells. These control cells were cultured without activators and express background levels of the phosphorylated target signal proteins. The Treated and Untreated Control Cells were fixed with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then washed with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) and lyophilized. Each vial contains a sufficient number of Treated or Untreated Control Cells for one multicolor stain using fluorescent antibodies specific for CD3, CD4, and CD8 and a single phosphorylated target protein.
Each lot of Treated Control Cells contains a measurable proportion of cells that express the specified phosphorylated signaling proteins. Representative BD Phosflow results are shown for typical staining of CD4+ and CD8+ Treated and Untreated Control Cells. Data from individual lots of these control cells may differ due to donor variation. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology, fluorescent antibody reagents and flow cytometers or cytometer settings.

Ingrédients: 5 vials of human treated and untreated control cells

Information d'application

Commentaires:

Lyophilized Cells

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: 4 °C

Référence

Perez, Mitchell, Campos, Gao, Li, Nolan: "Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry." dans: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Montag, Lotze: "Rapid flow cytometric measurement of cytokine-induced phosphorylation pathways [CIPP] in human peripheral blood leukocytes." dans: Clinical immunology (Orlando, Fla.), Vol. 121, Issue 2, pp. 215-26, (2006) (PubMed).

Perez, Mitchell, Jager, South, Murriel, McBride, Herzenberg, Kinoshita, Nolan: "Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1." dans: Nature immunology, Vol. 4, Issue 11, pp. 1083-92, (2003) (PubMed).