beta Actin Kit ELISA

N° du produit ABIN1568134

Détail du produit beta Actin Kit ELISA

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ACTb in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.63 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: ACTb (ACTB Produits)

Synonymes: BRWS1 Kit ELISA, PS1TP5BP1 Kit ELISA, ACTA1 Kit ELISA, Actx Kit ELISA, E430023M04Rik Kit ELISA, beta-actin Kit ELISA, ps1tp5bp1 Kit ELISA, act-2 Kit ELISA, actb Kit ELISA, B-actin Kit ELISA, LOC100174883 Kit ELISA, LOC100304816 Kit ELISA, A Kit ELISA, ACT5C Kit ELISA, Ac5C Kit ELISA, Act Kit ELISA, Act-5C Kit ELISA, Act5 Kit ELISA, Act5c Kit ELISA, Actin Kit ELISA, Actin/BAP47 Kit ELISA, Actin5C Kit ELISA, BAP47 Kit ELISA, Bap47 Kit ELISA, CG4027 Kit ELISA, Dmel\\CG4027 Kit ELISA, M32055 Kit ELISA, T11 Kit ELISA, act Kit ELISA, act 5C Kit ELISA, act5C Kit ELISA, actin Kit ELISA, actin5C Kit ELISA, anon-EST:fe2D2 Kit ELISA, beta-actin/Bap47 Kit ELISA, cyt5C Kit ELISA, l(1)G0009 Kit ELISA, l(1)G0010 Kit ELISA, l(1)G0025 Kit ELISA, l(1)G0079 Kit ELISA, l(1)G0117 Kit ELISA, l(1)G0177 Kit ELISA, l(1)G0245 Kit ELISA, l(1)G0330 Kit ELISA, l(1)G0420 Kit ELISA, l(1)G0486 Kit ELISA, Bact Kit ELISA, ACT-5 Kit ELISA, ACTB Kit ELISA, B-ACTZF Kit ELISA, actba Kit ELISA, bact Kit ELISA, bactin1 Kit ELISA, bactzf Kit ELISA, wu:fd18f05 Kit ELISA, A26C1B Kit ELISA, POTE2alpha Kit ELISA, POTEACTIN Kit ELISA, Beta-actin Kit ELISA, actin beta Kit ELISA, actin, beta Kit ELISA, cytoplasmic actin OlCA1 Kit ELISA, beta-actin Kit ELISA, Actin 5C Kit ELISA, actin gamma 1 Kit ELISA, actin, beta 1 Kit ELISA, actin, beta L homeolog Kit ELISA, beta actin Kit ELISA, POTE ankyrin domain family member F Kit ELISA, actin, beta like 2 Kit ELISA, actin, cytoplasmic 1 Kit ELISA, ACTB Kit ELISA, Actb Kit ELISA, actb Kit ELISA, LOC100174883 Kit ELISA, LOC100304816 Kit ELISA, Act5C Kit ELISA, ACTG1 Kit ELISA, actb1 Kit ELISA, actb.L Kit ELISA, LOC100136352 Kit ELISA, POTEF Kit ELISA, ACTBL2 Kit ELISA, LOC109073280 Kit ELISA

Sujet: Alternative name: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

ID gène: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction