COX IV Kit ELISA

N° du produit ABIN1569129

Détail du produit COX IV Kit ELISA

Antigène: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Épitope: Isoform 1

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of COX4I1 in human tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.058 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Autre désignation: COX4I1 (COX4I1 Produits)

Synonymes: COX4 Kit ELISA, COX4-1 Kit ELISA, COXIV Kit ELISA, mg:bb02d03 Kit ELISA, zgc:110058 Kit ELISA, GB20012 Kit ELISA, AL024441 Kit ELISA, Cox4 Kit ELISA, Cox4a Kit ELISA, cox4 Kit ELISA, cox4i1 Kit ELISA, coxiv Kit ELISA, BcDNA:AT07685 Kit ELISA, CG10396 Kit ELISA, COX IV Kit ELISA, CX41 Kit ELISA, CX42 Kit ELISA, Dmel\CG10396 Kit ELISA, IV Kit ELISA, cytochrome c oxidase subunit 4I1 Kit ELISA, cytochrome c oxidase subunit IV isoform 1 Kit ELISA, cytochrome c oxidase subunit 4 isoform 1, mitochondrial Kit ELISA, cytochrome c oxidase polypeptide IV Kit ELISA, cytochrome c oxidase subunit IV Kit ELISA, cytochrome c oxidase subunit 4i1 Kit ELISA, cytochrome c oxidase subunit IV isoform 1 S homeolog Kit ELISA, Cytochrome c oxidase subunit 4-like Kit ELISA, COX4I1 Kit ELISA, cox4i1 Kit ELISA, LOC412396 Kit ELISA, Cox4I1 Kit ELISA, LOC780848 Kit ELISA, BMEII0322 Kit ELISA, RSal33209_1582 Kit ELISA, SJAG_00976 Kit ELISA, HMPREF0733_11926 Kit ELISA, Cox4i1 Kit ELISA, cox4i1.S Kit ELISA, COX4L Kit ELISA

Sujet: Alternative name: COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

ID gène: 1327

UniProt: P13073

Pathways: Proton Transport