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Endothelin 2 Kit ELISA

EDN2 Reactivité: Rat Colorimetric Competition ELISA 7.812 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN1569528
  • Antigène Voir toutes Endothelin 2 (EDN2) Kits ELISA
    Endothelin 2 (EDN2)
    Reactivité
    • 6
    • 2
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    7.812 pg/mL - 500 pg/mL
    Seuil minimal de détection
    7.812 pg/mL
    Application
    ELISA
    Fonction
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of EDN2 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of this index.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    2.96 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Volume d'échantillon
    50 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C
    3. Aspirate and wash 3 times
    4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    5. Aspirate and wash 5 times
    6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    7. Add 50µL Stop Solution. Read at 450 nm immediately.
    Procédure de l'essai

    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

    Précision du teste
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Date de péremption
    12 months
  • Antigène Voir toutes Endothelin 2 (EDN2) Kits ELISA
    Endothelin 2 (EDN2)
    Autre désignation
    EDN2 (EDN2 Produits)
    Synonymes
    endothelin-2 Kit ELISA, EDN2 Kit ELISA, si:ch211-202b2.2 Kit ELISA, ET2 Kit ELISA, PPET2 Kit ELISA, VIC Kit ELISA, Et2 Kit ELISA, ET-2 Kit ELISA, endothelin 2 Kit ELISA, EDN2 Kit ELISA, edn2 Kit ELISA, Edn2 Kit ELISA
    Sujet
    Alternative name: ET2, PPET2, Preproendothelin-2
    ID gène
    24324
    UniProt
    P23943
    Pathways
    Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones
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