PCK1 Kit ELISA

N° du produit ABIN1571747

Détail du produit PCK1 Kit ELISA

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PCK1 in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.54 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PCK1 (PCK1 Produits)

Synonymes: PEPCK-C Kit ELISA, PEPCK1 Kit ELISA, PEPCKC Kit ELISA, PEPCK Kit ELISA, PEPCK-M Kit ELISA, PEPCK2 Kit ELISA, 1810010O14Rik Kit ELISA, 9130022B02Rik Kit ELISA, cb856 Kit ELISA, cb924 Kit ELISA, fj93h11 Kit ELISA, zgc:63869 Kit ELISA, wu:fc51c05 Kit ELISA, wu:fj93h11 Kit ELISA, pepck-c Kit ELISA, pepck1 Kit ELISA, pepckc Kit ELISA, PCK1 Kit ELISA, PPCK1 Kit ELISA, AI265463 Kit ELISA, Pck-1 Kit ELISA, GTP Kit ELISA, PCK Kit ELISA, Pepck Kit ELISA, RATPEPCK Kit ELISA, Ppc1C Kit ELISA, PHOSPHOENOLPYRUVATE CARBOXYKINASE Kit ELISA, T28I19.150 Kit ELISA, T28I19_150 Kit ELISA, phosphoenolpyruvate carboxykinase 1 Kit ELISA, 143299_at Kit ELISA, CG10924 Kit ELISA, CG17725 Kit ELISA, Dmel\\CG17725 Kit ELISA, Dromel_CG17725_FBtr0086701_pepck_mORF Kit ELISA, dPEPCK Kit ELISA, pepck Kit ELISA, PEPC Kit ELISA, PEPCase Kit ELISA, ppc Kit ELISA, phosphoenolpyruvate carboxykinase 1 Kit ELISA, phosphoenolpyruvate carboxykinase 2, mitochondrial Kit ELISA, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Kit ELISA, phosphoenolpyruvate carboxykinase 1 (soluble) Kit ELISA, phosphoenolpyruvate carboxykinase 1 S homeolog Kit ELISA, phosphoenolpyruvate carboxykinase, cytosolic [GTP] Kit ELISA, phosphoenolpyruvate carboxykinase 1, cytosolic Kit ELISA, phosphoenolpyruvate carboxylase 7 Kit ELISA, Phosphoenolpyruvate carboxykinase Kit ELISA, phosphoenolpyruvate carboxylase Kit ELISA, PCK1 Kit ELISA, PCK2 Kit ELISA, Pck2 Kit ELISA, pck1 Kit ELISA, pck1.S Kit ELISA, LOC100634531 Kit ELISA, Pck1 Kit ELISA, pep7 Kit ELISA, Pepck Kit ELISA, LOC107777405 Kit ELISA

Sujet: Alternative name: PEPCK1, PEPCKC, Cytosolic Phosphoenolpyruvate Carboxykinase, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

ID gène: 362282

UniProt: P07379

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis