Transferrin Kit ELISA
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- Antigène Voir toutes Transferrin (TF) Kits ELISA
- Transferrin (TF)
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Reactivité
- Chien
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 3.125 ng/mL - 200 ng/mL
- Seuil minimal de détection
- 3.125 ng/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TRF in canine serum, plasma and other biological fluids.
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of this index.
- Réactivité croisée (Details)
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- Sensibilité
- 1.12 ng/mL
- Ingrédients
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- Matériel non inclus
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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- Volume d'échantillon
- 100 μL
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - Procédure de l'essai
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The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- Restrictions
- For Research Use only
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- Précaution d'utilisation
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- Conseil sur la manipulation
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- Date de péremption
- 12 months
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- Antigène Voir toutes Transferrin (TF) Kits ELISA
- Transferrin (TF)
- Autre désignation
- TRF (TF Produits)
- Synonymes
- ltf Kit ELISA, pro1557 Kit ELISA, pro2086 Kit ELISA, mgc107777 Kit ELISA, LOC692564 Kit ELISA, TF Kit ELISA, LOC100144362 Kit ELISA, tf Kit ELISA, LTF Kit ELISA, TFEW Kit ELISA, conalbumin Kit ELISA, tf-b Kit ELISA, MGC64306 Kit ELISA, PRO1557 Kit ELISA, PRO2086 Kit ELISA, TFQTL1 Kit ELISA, AI266983 Kit ELISA, Cd176 Kit ELISA, HP Kit ELISA, Tf Kit ELISA, Tfn Kit ELISA, hpx Kit ELISA, Trf Kit ELISA, cb285 Kit ELISA, gavi Kit ELISA, id:ibd3238 Kit ELISA, id:ibd3525 Kit ELISA, sb:cb285 Kit ELISA, wu:fb57g06 Kit ELISA, wu:fb62h02 Kit ELISA, wu:fb63h10 Kit ELISA, wu:fb64h10 Kit ELISA, zgc:112154 Kit ELISA, 143958_at Kit ELISA, CG6186 Kit ELISA, Dmel\\CG6186 Kit ELISA, TSF1 Kit ELISA, anon-EST:Posey265 Kit ELISA, tsf1 Kit ELISA, Pro-TRH Kit ELISA, IL-5 Kit ELISA, STF I Kit ELISA, TRF1 Kit ELISA, sTF1 Kit ELISA, sTf Kit ELISA, tf1 Kit ELISA, transferrin Kit ELISA, serotransferrin Kit ELISA, transferrin (ovotransferrin) Kit ELISA, transferrin L homeolog Kit ELISA, melanotransferrin Kit ELISA, transferrin-a Kit ELISA, Transferrin 1 Kit ELISA, thyrotropin releasing hormone Kit ELISA, interleukin 5 Kit ELISA, TF Kit ELISA, LOC477072 Kit ELISA, tf Kit ELISA, Tf Kit ELISA, LOC100144362 Kit ELISA, tf.L Kit ELISA, LOC5575625 Kit ELISA, Trf Kit ELISA, tfa Kit ELISA, Tsf1 Kit ELISA, TRH Kit ELISA, IL5 Kit ELISA, LOC101085148 Kit ELISA, LOC100726872 Kit ELISA, trf Kit ELISA
- Sujet
- Alternative name: TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin
- Pathways
- Transition Metal Ion Homeostasis
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