(1 reference)
Aperçu rapide pour CellCountEZ™ Cell Survival Assay Kit (ABIN1607918)
Application
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N° du produit (Fournisseur)
- kld-001
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Fournisseur
- Rockland
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Marque
- CellCountEZ™
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivité: Souris Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 Reactivité: Humain Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 Reactivité: Humain Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReactivité: Différentes espèces Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivité: Souris Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Interleukin 6 (IL6) ELISA KitIL6 Reactivité: Humain Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivité: Humain Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
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Indications d'application
- CellCountEZ™ Cell Survival Assay Kit contains enough reagents for 1000 assays using a 96-well plate. This assay gives a linear response for cells (0, 1.000, 5.000, 10.000, 20.000, 40.000) plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate and measured 20 hours after plating. Drug toxicity could be measured for up to 4 days after treatment with drugs using this assay for 5.000 cells plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store kit at 2-8° C prior to opening. See kit insert for complete instructions.
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Date de péremption
- See kit insert for complete instructions.
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: "Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy." dans: Oncotarget, Vol. 7, Issue 16, pp. 21812-24, (2017) (PubMed).
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: "Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy." dans: Oncotarget, Vol. 7, Issue 16, pp. 21812-24, (2017) (PubMed).
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Sujet
- CellCountEZ™ Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods, this preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly before transport into the extracellular culture media. CellCountEZ™ offers many advantages that make it superior to common existing methods for quantifying cell growth and survival. Synonyms: Toxicity, Proliferation, and Survival Assay, cell proliferation and survival, drug screening, cell viability, cell survival, cellular viability, cellular survival, HEDS assay.
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