CCL24 Kit ELISA

N° du produit ABIN1672724

Détail du produit CCL24 Kit ELISA

Antigène: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Épitope: AA 27-119

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78-5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL24/Eotaxin-2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: V27-C119

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: V27-C119

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Tissue Specificity: Activated monocytes and activated T lymphocytes.

Plaque: Pre-coated

Protocole: human CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli, V27-C119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL24 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CCL24 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL24 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 525, Standard deviation: 40.95, CV(%): 7.8
• Sample 2: n=16, Mean(pg/ml): 1357, Standard deviation: 97.7, CV(%): 7.2
• Sample 3: n=16, Mean(pg/ml): 2556, Standard deviation: 158.5, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 630, Standard deviation: 55.44, CV(%): 8.8
• Sample 2: n=24, Mean(pg/ml): 1749, Standard deviation: 129.4, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 3018, Standard deviation: 268.6, CV(%): 8.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Autre désignation: CCL24 (CCL24 Produits)

Synonymes: Ckb-6 Kit ELISA, MPIF-2 Kit ELISA, MPIF2 Kit ELISA, SCYA24 Kit ELISA, CKb-6 Kit ELISA, Scya24 Kit ELISA, C-C motif chemokine ligand 24 Kit ELISA, chemokine (C-C motif) ligand 24 Kit ELISA, CCL24 Kit ELISA, Ccl24 Kit ELISA

Sujet:

Protein Function: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.

Background: Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Synonyms: C-C motif chemokine 24,CK-beta-6,Eosinophil chemotactic protein 2,Eotaxin-2,Myeloid progenitor inhibitory factor 2,MPIF-2,Small-inducible cytokine A24,CCL24,MPIF2, SCYA24,

Full Gene Name: C-C motif chemokine 24

Cellular Localisation: Secreted.

ID gène: 6369

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization