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CCL7 Kit ELISA

CCL7 Reactivité: Humain AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672742
  • Antigène Voir toutes CCL7 Kits ELISA
    CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))
    Épitope
    AA 24-99
    Reactivité
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL7/MCP-3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: Q24-L99
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <1pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: Q24-L99
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Plaque
    Pre-coated
    Protocole
    human CCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL7 has been precoated onto 96-well plates. Standards(E.coli, Q24-L99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL7 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL7 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 154, Standard deviation: 6.78, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 296, Standard deviation: 14.8, CV(%): 5
    • Sample 3: n=16, Mean(pg/ml): 577, Standard deviation: 35.2, CV(%): 6.1,
    • Sample 1: n=24, Mean(pg/ml): 166, Standard deviation: 11.3, CV(%): 6.8
    • Sample 2: n=24, Mean(pg/ml): 325, Standard deviation: 22.43, CV(%): 6.9
    • Sample 3: n=24, Mean(pg/ml): 593, Standard deviation: 42.7, CV(%): 7.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Zhao, Bu, Wang, Ma, Ma: "GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation." dans: PLoS ONE, Vol. 9, Issue 1, pp. e85058, (2014) (PubMed).

  • Antigène Voir toutes CCL7 Kits ELISA
    CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))
    Autre désignation
    CCL7 (CCL7 Produits)
    Synonymes
    CCL7 Kit ELISA, FIC Kit ELISA, MARC Kit ELISA, MCP-3 Kit ELISA, MCP3 Kit ELISA, NC28 Kit ELISA, SCYA6 Kit ELISA, SCYA7 Kit ELISA, Scya7 Kit ELISA, fic Kit ELISA, marc Kit ELISA, mcp3 Kit ELISA, C-C motif chemokine ligand 7 Kit ELISA, chemokine (C-C motif) ligand 7 Kit ELISA, CCL7 Kit ELISA, Ccl7 Kit ELISA
    Sujet

    Protein Function: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

    Background: Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

    Synonyms: C-C motif chemokine 7,Monocyte chemoattractant protein 3,Monocyte chemotactic protein 3,MCP-3,NC28,Small-inducible cytokine A7,CCL7,MCP3, SCYA6, SCYA7,

    Full Gene Name: C-C motif chemokine 7

    Cellular Localisation: Secreted.
    ID gène
    6354
    UniProt
    P80098
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