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P-Cadherin Kit ELISA

CDH3 Reactivité: Souris AA 100-647 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN1672767
  • Antigène Voir toutes P-Cadherin (CDH3) Kits ELISA
    P-Cadherin (CDH3)
    Épitope
    AA 100-647
    Reactivité
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse P-Cadherin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: E100-G647
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: E100-G647
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse P-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for P-Cadherin has been precoated onto 96-well plates. Standards(NSO, E100-G647) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Cadherin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse P-Cadherin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 433, Standard deviation: 27.28, CV(%): 6.3
    • Sample 2: n=16, Mean(pg/ml): 1314, Standard deviation: 88.04, CV(%): 6.7
    • Sample 3: n=16, Mean(pg/ml): 2658, Standard deviation: 180.74, CV(%): 6.8,
    • Sample 1: n=24, Mean(pg/ml): 437, Standard deviation: 31.90, CV(%): 7.3
    • Sample 2: n=24, Mean(pg/ml): 1356, Standard deviation: 103.06, CV(%): 7.6
    • Sample 3: n=24, Mean(pg/ml): 2713, Standard deviation: 211.61, CV(%): 7.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes P-Cadherin (CDH3) Kits ELISA
    P-Cadherin (CDH3)
    Autre désignation
    CDH3 (CDH3 Produits)
    Synonymes
    CDH3 Kit ELISA, Cadherin-3 Kit ELISA, AI385538 Kit ELISA, Cadp Kit ELISA, Cdhp Kit ELISA, P-cadherin Kit ELISA, Pcad Kit ELISA, CDHP Kit ELISA, HJMD Kit ELISA, PCAD Kit ELISA, E-Cad Kit ELISA, E-cadherin Kit ELISA, EP-cadherin Kit ELISA, XTCAD-1 Kit ELISA, c-cadherin Kit ELISA, cadherin-1 Kit ELISA, cdh1 Kit ELISA, cdh1-b Kit ELISA, cdh3 Kit ELISA, cdhc-A Kit ELISA, cdhp Kit ELISA, hjmd Kit ELISA, l-cam Kit ELISA, lcam Kit ELISA, pcad Kit ELISA, uvo Kit ELISA, uvomorulin Kit ELISA, xb-cad Kit ELISA, xcdh1 Kit ELISA, CDH15 Kit ELISA, K-CAM Kit ELISA, cadherin 3 Kit ELISA, cadherin 3 L homeolog Kit ELISA, Cadherin-3 Kit ELISA, CDH3 Kit ELISA, Cdh3 Kit ELISA, cdh3.L Kit ELISA, cdh-3 Kit ELISA
    Sujet

    Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

    Background: P-Cadherin, also known as Cadherin-3(CDH3), is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. Cells expressing CDH3 also adhered to one another more tightly than the parental cell line.

    Synonyms: Cadherin-3,Placental cadherin,P-cadherin,Cdh3,Cdhp,

    Full Gene Name: Cadherin-3

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein.
    ID gène
    12560
    UniProt
    P10287
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