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Cathepsin D Kit ELISA

CTSD Reactivité: Souris AA 21-410 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672768
  • Antigène Voir toutes Cathepsin D (CTSD) Kits ELISA
    Cathepsin D (CTSD)
    Épitope
    AA 21-410
    Reactivité
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cathepsin D
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: I21-L410
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: I21-L410
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin D has been precoated onto 96-well plates. Standards(NSO, I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin D amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Cathepsin D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Cathepsin D standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.13, Standard deviation: 0.033, CV(%): 2.9
    • Sample 2: n=16, Mean(ng/ml): 3.29, Standard deviation: 0.115, CV(%): 3.5
    • Sample 3: n=16, Mean(ng/ml): 6.68, Standard deviation: 0.247, CV(%): 3.7,
    • Sample 1: n=24, Mean(ng/ml): 1.19, Standard deviation: 0.043, CV(%): 3.6
    • Sample 2: n=24, Mean(ng/ml): 3.34, Standard deviation: 0.134, CV(%): 4
    • Sample 3: n=24, Mean(ng/ml): 6.73, Standard deviation: 0.283, CV(%): 4.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Cathepsin D (CTSD) Kits ELISA
    Cathepsin D (CTSD)
    Autre désignation
    CTSD (CTSD Produits)
    Synonymes
    catD Kit ELISA, fb93e11 Kit ELISA, fj17b09 Kit ELISA, wu:fb93e11 Kit ELISA, wu:fj17b09 Kit ELISA, LOC398557 Kit ELISA, Cathd Kit ELISA, CatD Kit ELISA, LOC100136761 Kit ELISA, CTSD Kit ELISA, DKFZp469J0315 Kit ELISA, DDBDRAFT_0205785 Kit ELISA, DDBDRAFT_0215012 Kit ELISA, DDB_0205785 Kit ELISA, DDB_0215012 Kit ELISA, CLN10 Kit ELISA, CPSD Kit ELISA, CD Kit ELISA, cathepsin D Kit ELISA, cathepsin D S homeolog Kit ELISA, lysosomal aspartic protease Kit ELISA, cathepsin D-like Kit ELISA, ctsd Kit ELISA, LOC398557 Kit ELISA, ctsd.S Kit ELISA, CatD Kit ELISA, LOC5567565 Kit ELISA, LOC100136761 Kit ELISA, CTSD Kit ELISA, Cathd Kit ELISA, ctsD Kit ELISA, catd Kit ELISA, Ctsd Kit ELISA, LOC443060 Kit ELISA
    Sujet

    Protein Function: Acid protease active in intracellular protein breakdown.

    Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

    Synonyms: Cathepsin D,3.4.23.5,Ctsd,

    Full Gene Name: Cathepsin D

    Cellular Localisation: Lysosome. Melanosome . Secreted, extracellular space.
    ID gène
    13033
    UniProt
    P18242
    Pathways
    Peptide Hormone Metabolism
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