CTLA4 Kit ELISA

N° du produit ABIN1672780

Détail du produit CTLA4 Kit ELISA

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Épitope: AA 37-162

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CTLA4

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A37-F162

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A37-F162

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Widely expressed with highest levels in lymphoid tissues. .

Plaque: Pre-coated

Protocole: mouse CTLA4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CTLA4 has been precoated onto 96-well plates. Standards(NSO, A37-F162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CTLA4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CTLA4 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CTLA4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CTLA4 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.42, Standard deviation: 0.051, CV(%): 3.6
• Sample 2: n=16, Mean(ng/ml): 3.53, Standard deviation: 0.138, CV(%): 3.9
• Sample 3: n=16, Mean(ng/ml): 6.82, Standard deviation: 0.286, CV(%): 4.2,
• Sample 1: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.063, CV(%): 4.3
• Sample 2: n=24, Mean(ng/ml): 3.71, Standard deviation: 0.171, CV(%): 4.6
• Sample 3: n=24, Mean(ng/ml): 6.87, Standard deviation: 0.426, CV(%): 6.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Autre désignation: CTLA4 (CTLA4 Produits)

Synonymes: CTLA-4 Kit ELISA, CTLA4 Kit ELISA, CD152 Kit ELISA, Cd152 Kit ELISA, Ctla-4 Kit ELISA, Ly-56 Kit ELISA, CD Kit ELISA, CELIAC3 Kit ELISA, GRD4 Kit ELISA, GSE Kit ELISA, IDDM12 Kit ELISA, sCTLA4 Kit ELISA, cytotoxic T-lymphocyte associated protein 4 Kit ELISA, cytotoxic T-lymphocyte-associated protein 4 Kit ELISA, CTLA4 Kit ELISA, Ctla4 Kit ELISA

Sujet:

Protein Function: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (By similarity). .

Background: CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Synonyms: Cytotoxic T-lymphocyte protein 4,Cytotoxic T-lymphocyte-associated antigen 4,CTLA-4,CD152,Ctla4,Cd152,

Full Gene Name: Cytotoxic T-lymphocyte protein 4

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 12477

UniProt: P09793

Pathways: Cancer Immune Checkpoints