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Decorin Kit ELISA

DCN Reactivité: Humain AA 31-359 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672790
  • Antigène Voir toutes Decorin (DCN) Kits ELISA
    Decorin (DCN)
    Épitope
    AA 31-359
    Reactivité
    • 8
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Decorin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: D31-K359
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: D31-K359
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

    Plaque
    Pre-coated
    Protocole
    human Decorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Decorin has been precoated onto 96-well plates. Standards(NSO, D31-K359) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Decorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Decorin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Decorin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Decorin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 128, Standard deviation: 5.76, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 645, Standard deviation: 41.28, CV(%): 6.4
    • Sample 3: n=16, Mean(pg/ml): 1274, Standard deviation: 72.62, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 139, Standard deviation: 8.48, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 677, Standard deviation: 56.9, CV(%): 8.4
    • Sample 3: n=24, Mean(pg/ml): 1333, Standard deviation: 89.31, CV(%): 6.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Decorin (DCN) Kits ELISA
    Decorin (DCN)
    Autre désignation
    DCN (DCN Produits)
    Synonymes
    CSCD Kit ELISA, DSPG2 Kit ELISA, PG40 Kit ELISA, PGII Kit ELISA, PGS2 Kit ELISA, SLRR1B Kit ELISA, DC Kit ELISA, mDcn Kit ELISA, cscd Kit ELISA, dspg2 Kit ELISA, pg40 Kit ELISA, pgii Kit ELISA, pgs2 Kit ELISA, slrr1b Kit ELISA, decorin Kit ELISA, decorin L homeolog Kit ELISA, DCN Kit ELISA, Dcn Kit ELISA, dcn.L Kit ELISA, dcn Kit ELISA
    Sujet

    Protein Function: May affect the rate of fibrils formation.

    Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size.It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

    Synonyms: Decorin,Bone proteoglycan II,PG-S2,PG40,DCN,SLRR1B,

    Full Gene Name: Decorin

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    ID gène
    1634
    UniProt
    P07585
    Pathways
    Glycosaminoglycan Metabolic Process
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