Thrombospondin 1 Kit ELISA

N° du produit ABIN1672826

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Thrombospondin 1.

Aperçu rapide pour Thrombospondin 1 Kit ELISA (ABIN1672826)

Antigène: Thrombospondin 1 (THBS1)

Épitope: AA 19-1170

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit Thrombospondin 1 Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human THBS1/TSP1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: N19-P1170

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <50pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: N19-P1170

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the thrombospondin family.

Plaque: Pre-coated

Protocole: human THBS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been precoated onto 96-well plates. Standards(NSO, N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for THBS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human THBS1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 15.5, Standard deviation: 0.93, CV(%): 6
• Sample 2: n=16, Mean(ng/ml): 32, Standard deviation: 1.76, CV(%): 5.5
• Sample 3: n=16, Mean(ng/ml): 52, Standard deviation: 2.08, CV(%): 4,
• Sample 1: n=24, Mean(ng/ml): 16.3, Standard deviation: 1.27, CV(%): 7.8
• Sample 2: n=24, Mean(ng/ml): 38, Standard deviation: 2.47, CV(%): 6.5
• Sample 3: n=24, Mean(ng/ml): 64, Standard deviation: 4.61, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Thrombospondin 1 (THBS1)

Autre désignation: THBS1

Sujet:

Protein Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). .

Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Synonyms: Thrombospondin-1,THBS1,TSP, TSP1,

Full Gene Name: Thrombospondin-1

Cellular Localisation: Endoplasmic reticulum . Sarcoplasmic reticulum.

ID gène: 7057

UniProt: P07996

Pathways: Autophagy