CD103, Intestinal Lymphocyte (HML1) 105-150 kDa Kit ELISA

N° du produit ABIN1672842

Le kit ELISA CD103, Intestinal Lymphocyte (HML1) 105-150 kDa (ABIN1672842) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CD103, Intestinal Lymphocyte (HML1) 105-150 kDa. Ce kit ELISA a été cité dans 3 publications.

Aperçu rapide pour CD103, Intestinal Lymphocyte (HML1) 105-150 kDa Kit ELISA (ABIN1672842)

Antigène: CD103, Intestinal Lymphocyte (HML1) 105-150 kDa

Épitope: AA 33-251

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Cell Lysate, Urine

Détail du produit

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Human Tissue Factor/F3 ELISA Kit to quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: S33-E251

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.5%, 6.6%, 7.6%
Inter-Assay Precision (CV%): 5.5%, 6.9%, 8.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Référence

Cohen, Prendergast, Geddings, Walts, Agadjanian, Hisada, Karlan, Mackman, Walsh: "Evaluation of venous thrombosis and tissue factor in epithelial ovarian cancer." dans: Gynecologic oncology, Vol. 146, Issue 1, pp. 146-152, (2017) (PubMed).

Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." dans: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

Chen, Wei, Zhuang, Qiao, Tang, Zhang, Wei, Fang, Chen, Du, Huang, Jiang, Hu, Hua: "Proteomic screening of anaerobically regulated promoters from Salmonella and its antitumor applications." dans: Molecular & cellular proteomics : MCP, Vol. 10, Issue 6, pp. M111.009399, (2011) (PubMed).

Détail du antigène

Antigène: CD103, Intestinal Lymphocyte (HML1) 105-150 kDa

Autre désignation: Tissue factor/F2

Sujet:

Background: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Gene Full Name: coagulation factor III, tissue factor

ID gène: 2152

UniProt: P13726