IL-22 Kit ELISA

N° du produit ABIN1672846

Détail du produit IL-22 Kit ELISA

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Épitope: AA 34-179

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-22

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A34-I179

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A34-I179

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the IL-10 family.

Plaque: Pre-coated

Protocole: human IL-22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-22 has been precoated onto 96-well plates. Standards(E.coli, A34-I179) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-22 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-22 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-22 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 74, Standard deviation: 3.63, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 312, Standard deviation: 18.1, CV(%): 5.8
• Sample 3: n=16, Mean(pg/ml): 611, Standard deviation: 37.3, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 87, Standard deviation: 4.7, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 386, Standard deviation: 25.5, CV(%): 6.6
• Sample 3: n=24, Mean(pg/ml): 695, Standard deviation: 48, CV(%): 6.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-22 Kit ELISA (ABIN1672846)

Min, Yan, Wang, Landeck, Chen, Li, Cai, Zheng, Man: "Rottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies." dans: PLoS ONE, Vol. 12, Issue 12, pp. e0190051, (2018) (PubMed).

Chai, Yang, Gao, Guo, Li, Fan, Wu, Xue, Cai, Jiang, Qin, Zhang, Ke: "Decreased percentages of regulatory T cells are necessary to activate Th1-Th17-Th22 responses during acute rejection of the peripheral nerve xenotransplantation in mice." dans: Transplantation, Vol. 98, Issue 7, pp. 729-37, (2014) (PubMed).

Détail du antigène

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Autre désignation: IL22 (IL22 Produits)

Synonymes: IL-21 Kit ELISA, IL-22 Kit ELISA, IL-D110 Kit ELISA, IL-TIF Kit ELISA, ILTIF Kit ELISA, TIFIL-23 Kit ELISA, TIFa Kit ELISA, zcyto18 Kit ELISA, IL-22a Kit ELISA, ILTIFa Kit ELISA, Iltif Kit ELISA, RGD1561292 Kit ELISA, interleukin-22 Kit ELISA, interleukin 22 Kit ELISA, IL22 Kit ELISA, il22 Kit ELISA, Il22 Kit ELISA

Sujet:

Protein Function: Cytokine that contributes to the inflammatory response in vivo.

Background: Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Synonyms: Interleukin-22,IL-22,Cytokine Zcyto18,IL-10-related T-cell-derived-inducible factor,IL-TIF,IL22,ILTIF, ZCYTO18,UNQ3099/PRO10096,

Full Gene Name: Interleukin-22

Cellular Localisation: Secreted.

ID gène: 50616

UniProt: Q9GZX6