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PROCR Kit ELISA

PROCR Reactivité: Humain AA 18-210 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672861
  • Antigène Voir toutes PROCR Kits ELISA
    PROCR (Protein C Receptor, Endothelial (PROCR))
    Épitope
    AA 18-210
    Reactivité
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    312-20000 pg/mL
    Seuil minimal de détection
    312 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human EPCR
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: S18-S210
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: S18-S210
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

    Plaque
    Pre-coated
    Protocole
    human EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCR has been precoated onto 96-well plates. Standards(NSO, S18-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCR amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human EPCR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EPCR standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 2.21, Standard deviation: 0.095, CV(%): 4.3
    • Sample 2: n=16, Mean(ng/ml): 8.45, Standard deviation: 0.313, CV(%): 3.7
    • Sample 3: n=16, Mean(ng/ml): 14.4, Standard deviation: 0.691, CV(%): 4.8,
    • Sample 1: n=24, Mean(ng/ml): 2.13, Standard deviation: 0.124, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 8.79, Standard deviation: 0.466, CV(%): 5.3
    • Sample 3: n=24, Mean(ng/ml): 13.7, Standard deviation: 1.04, CV(%): 7.6
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes PROCR Kits ELISA
    PROCR (Protein C Receptor, Endothelial (PROCR))
    Autre désignation
    PROCR (PROCR Produits)
    Synonymes
    PROCR Kit ELISA, CCCA Kit ELISA, CCD41 Kit ELISA, EPCR Kit ELISA, AI325044 Kit ELISA, Ccca Kit ELISA, Ccd41 Kit ELISA, Epcr Kit ELISA, protein C receptor Kit ELISA, protein C receptor, endothelial Kit ELISA, PROCR Kit ELISA, Procr Kit ELISA
    Sujet

    Protein Function: Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex, plays a role in the protein C pathway controlling blood coagulation.

    Background: Endothelial protein C receptor(EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

    Synonyms: Endothelial protein C receptor,Activated protein C receptor,APC receptor,Endothelial cell protein C receptor,CD201,PROCR,EPCR,

    Full Gene Name: Endothelial protein C receptor

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    10544
    UniProt
    Q9UNN8
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