Thymic Stromal Lymphopoietin Kit ELISA

N° du produit ABIN1672862

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Thymic Stromal Lymphopoietin.

Aperçu rapide pour Thymic Stromal Lymphopoietin Kit ELISA (ABIN1672862)

Antigène: Thymic Stromal Lymphopoietin (TSLP)

Épitope: AA 29-159

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit Thymic Stromal Lymphopoietin Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TSLP

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: Y29-Q159

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: Y29-Q159

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. .

Plaque: Pre-coated

Protocole: human TSLP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TSLP has been precoated onto 96-well plates. Standards(E.coli, Y29-Q159) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSLP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TSLP amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TSLP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TSLP standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 258, Standard deviation: 23.99, CV(%): 9.3
• Sample 2: n=16, Mean(pg/ml): 762, Standard deviation: 57.15, CV(%): 7.5
• Sample 3: n=16, Mean(pg/ml): 1229, Standard deviation: 103.24, CV(%): 8.4,
• Sample 1: n=24, Mean(pg/ml): 262, Standard deviation: 25.41, CV(%): 9.7
• Sample 2: n=24, Mean(pg/ml): 770, Standard deviation: 58.52, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 1335, Standard deviation: 117.48, CV(%): 8.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Thymic Stromal Lymphopoietin (TSLP)

Autre désignation: TSLP

Sujet:

Protein Function: Isoform 1: Cytokine that induces the release of T-cell- attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. .

Background: Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.

Synonyms: Thymic stromal lymphopoietin,TSLP,

Full Gene Name: Thymic stromal lymphopoietin

Cellular Localisation: Secreted.

ID gène: 85480

UniProt: Q969D9