Granulin Kit ELISA

N° du produit ABIN1672874

Détail du produit Granulin Kit ELISA

Antigène: Granulin (GRN)

Épitope: AA 18-593

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Progranulin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: T18-L593

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <50pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: T18-L593

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the granulin family.

Tissue Specificity: In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

Plaque: Pre-coated

Protocole: human Progranulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Progranulin has been precoated onto 96-well plates. Standards(NSO, T18-L593) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Progranulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Progranulin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Progranulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Progranulin standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 15.4, Standard deviation: 0.986, CV(%): 6.4
• Sample 2: n=16, Mean(ng/ml): 37.7, Standard deviation: 2, CV(%): 5.3
• Sample 3: n=16, Mean(ng/ml): 56, Standard deviation: 2.69, CV(%): 4.8,
• Sample 1: n=24, Mean(ng/ml): 13.6, Standard deviation: 1.02, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 32.8, Standard deviation: 2.69, CV(%): 8.2
• Sample 3: n=24, Mean(ng/ml): 61, Standard deviation: 4.76, CV(%): 7.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Granulin (GRN)

Autre désignation: GRN (GRN Produits)

Synonymes: GRN Kit ELISA, grn Kit ELISA, wu:fb11g12 Kit ELISA, wu:fj18f05 Kit ELISA, MGC53354 Kit ELISA, DKFZp459H066 Kit ELISA, CLN11 Kit ELISA, GEP Kit ELISA, GP88 Kit ELISA, PCDGF Kit ELISA, PEPI Kit ELISA, PGRN Kit ELISA, epithelin Kit ELISA, acrogranin Kit ELISA, granulin precursor Kit ELISA, granulin a Kit ELISA, Granulins Kit ELISA, granulin L homeolog Kit ELISA, granulin Kit ELISA, GRN Kit ELISA, grna Kit ELISA, grn Kit ELISA, grn.L Kit ELISA, granulin Kit ELISA, ORF1 granulin Kit ELISA, Grn Kit ELISA

Classe de substances: Viral Protein

Sujet:

Protein Function: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.

Background: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Synonyms: Granulins,Proepithelin,PEPI,Acrogranin,Glycoprotein of 88 Kda,GP88,Glycoprotein 88,Progranulin,Paragranulin,Granulin-1,Granulin G,Granulin-2,Granulin F,Granulin-3,Granulin B,Granulin-4,Granulin A,Granulin-5,Granulin C,Granulin-6,Granulin D,Granulin-7,Granulin E,GRN,

Full Gene Name: Granulins

Cellular Localisation: Secreted.

ID gène: 2896

UniProt: P28799