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CHI3L1 Kit ELISA

CHI3L1 Reactivité: Humain AA 22-383 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
N° du produit ABIN1672875
  • Antigène Voir toutes CHI3L1 Kits ELISA
    CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))
    Épitope
    AA 22-383
    Reactivité
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Chitinase 3-like 1/YKL-40
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: Y22-T383
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: Y22-T383
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the glycosyl hydrolase 18 family.

    Tissue Specificity: Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. .

    Plaque
    Pre-coated
    Protocole
    human Chitinase 3-like 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chitinase 3-like 1 has been precoated onto 96-well plates. Standards(NSO, Y22-T383) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chitinase 3-like 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Chitinase 3-like 1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Chitinase 3-like 1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Chitinase 3-like 1 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 562, Standard deviation: 28.7, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 1059, Standard deviation: 51.9, CV(%): 4.9
    • Sample 3: n=16, Mean(pg/ml): 2325, Standard deviation: 123, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 643, Standard deviation: 43.1, CV(%): 6.7
    • Sample 2: n=24, Mean(pg/ml): 1124, Standard deviation: 71.9, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 2239, Standard deviation: 165, CV(%): 7.4
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CHI3L1 Kits ELISA
    CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))
    Autre désignation
    CHI3L1 (CHI3L1 Produits)
    Synonymes
    AW208766 Kit ELISA, Brp39 Kit ELISA, Gp39 Kit ELISA, ASRT7 Kit ELISA, CGP-39 Kit ELISA, GP-39 Kit ELISA, GP39 Kit ELISA, HC-gp39 Kit ELISA, HCGP-3P Kit ELISA, YKL-40 Kit ELISA, YKL40 Kit ELISA, YYL-40 Kit ELISA, hCGP-39 Kit ELISA, GP38K Kit ELISA, CLP-1 Kit ELISA, SPC-40 Kit ELISA, SPS-40 Kit ELISA, Chil1 Kit ELISA, BP40 Kit ELISA, MGP-40 Kit ELISA, chitinase-like 1 Kit ELISA, chitinase 3 like 1 Kit ELISA, chitinase 3-like 1 (cartilage glycoprotein-39) Kit ELISA, Chil1 Kit ELISA, CHI3L1 Kit ELISA, Chi3l1 Kit ELISA
    Classe de substances
    Viral Protein
    Sujet

    Protein Function: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. .

    Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40 kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

    Synonyms: Chitinase-3-like protein 1,39 kDa synovial protein,Cartilage glycoprotein 39,CGP-39,GP-39,hCGP-39,YKL-40,CHI3L1,

    Full Gene Name: Chitinase-3-like protein 1

    Cellular Localisation: Secreted, extracellular space . Cytoplasm . Cytoplasm, perinuclear region . Endoplasmic reticulum.
    ID gène
    1116
    UniProt
    P36222
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