CRP Kit ELISA

N° du produit ABIN1672879

Détail du produit CRP Kit ELISA

Antigène: CRP (C-Reactive Protein (CRP))

Épitope: AA 20-230

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CRP

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: H20-S230

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: H20-S230

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the pentaxin family.

Tissue Specificity: Found in plasma.

Plaque: Pre-coated

Protocole: rat CRP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRP has been precoated onto 96-well plates. Standards(NSO, H20-S230) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CRP amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat CRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CRP standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1462, Standard deviation: 99.4, CV(%): 6.8
• Sample 2: n=16, Mean(pg/ml): 2359, Standard deviation: 146.3, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 5759, Standard deviation: 264.9, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 1478, Standard deviation: 124.2, CV(%): 8.4
• Sample 2: n=24, Mean(pg/ml): 2348, Standard deviation: 166.7, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 5879, Standard deviation: 399.8, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CRP Kit ELISA (ABIN1672879)

Liao, Zheng, Xie, Zhao, Zeng, Liu, Liu: "Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats." dans: Stem cell research & therapy, Vol. 8, Issue 1, pp. 286, (2018) (PubMed).

Zhao, Han: "Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy." dans: Medical science monitor basic research, Vol. 24, pp. 84-92, (2018) (PubMed).

Zou, Huang, Lu, Fang, Wang, Zhao, Jia, Xu, Wang, Wang, Dong: "The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation." dans: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 165, (2017) (PubMed).

Li, Su, Xu, Huang, Xu: "Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides." dans: Neuropsychiatric disease and treatment, Vol. 13, pp. 2477-2487, (2017) (PubMed).

Wang, Chai, He, Ai, Sun, Huang, Li: "Intermittent hypoxia simulating obstructive sleep apnea causes pulmonary inflammation and activates the Nrf2/HO-1 pathway." dans: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3463-3470, (2017) (PubMed).

Lu, Li, Li, Wang, Wu, Li, Wang: "Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1359105, (2016) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." dans: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Shi, Song, Zhang, Li, Li: "Correlation between the microinflammatory state and left ventricular structural and functional changes in maintenance haemodialysis patients." dans: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 532-536, (2013) (PubMed).

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." dans: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Détail du antigène

Antigène: CRP (C-Reactive Protein (CRP))

Autre désignation: C-Reactive Protein (CRP Produits)

Synonymes: PTX1 Kit ELISA, crp Kit ELISA, AI255847 Kit ELISA, Aa1249 Kit ELISA, Ab1-341 Kit ELISA, Ab2-196 Kit ELISA, Ac1-114 Kit ELISA, Ac1262 Kit ELISA, Ac2-069 Kit ELISA, Ba2-693 Kit ELISA, APCS Kit ELISA, 0610010I23Rik Kit ELISA, AW743261 Kit ELISA, C77570 Kit ELISA, CRP2 Kit ELISA, CRP4 Kit ELISA, Crp Kit ELISA, ESP1 Kit ELISA, Hlp Kit ELISA, CRP5.1 Kit ELISA, zgc:152809 Kit ELISA, C-reactive protein Kit ELISA, C-reactive protein, pentraxin-related Kit ELISA, c-reactive protein, pentraxin-related Kit ELISA, cysteine rich protein 2 Kit ELISA, CRP Kit ELISA, crp Kit ELISA, Crp Kit ELISA, Crip2 Kit ELISA, LOC776376 Kit ELISA

Sujet:

Protein Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity). .

Background: C Reactive Protein(CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Synonyms: C-reactive protein,Crp,Ptx1,

Full Gene Name: C-reactive protein

Cellular Localisation: Secreted.

ID gène: 25419

UniProt: P48199

Pathways: Carbohydrate Homeostasis