CRP Kit ELISA

N° du produit ABIN1672879

Kit ELISA CRP Rat, Colorimetric test pour la quantification de Rat CRP et a été mentionné dans 9+ publications.

Aperçu rapide pour CRP Kit ELISA (ABIN1672879)

Antigène: CRP (C-Reactive Protein (CRP))

Épitope: AA 20-230

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit CRP Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Rat CRP/C Reactive Protein ELISA Kit to quantitate Rat Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: H20-S230

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.9%, 5.4%, 6.4%
Inter-Assay Precision (CV%): 5%, 7.2%, 7.4%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour CRP Kit ELISA (ABIN1672879)

Liao, Zheng, Xie, Zhao, Zeng, Liu, Liu: "Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats." dans: Stem cell research & therapy, Vol. 8, Issue 1, pp. 286, (2018) (PubMed).

Zhao, Han: "Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy." dans: Medical science monitor basic research, Vol. 24, pp. 84-92, (2018) (PubMed).

Zou, Huang, Lu, Fang, Wang, Zhao, Jia, Xu, Wang, Wang, Dong: "The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation." dans: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 165, (2017) (PubMed).

Li, Su, Xu, Huang, Xu: "Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides." dans: Neuropsychiatric disease and treatment, Vol. 13, pp. 2477-2487, (2017) (PubMed).

Wang, Chai, He, Ai, Sun, Huang, Li: "Intermittent hypoxia simulating obstructive sleep apnea causes pulmonary inflammation and activates the Nrf2/HO-1 pathway." dans: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3463-3470, (2017) (PubMed).

Lu, Li, Li, Wang, Wu, Li, Wang: "Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1359105, (2016) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." dans: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Shi, Song, Zhang, Li, Li: "Correlation between the microinflammatory state and left ventricular structural and functional changes in maintenance haemodialysis patients." dans: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 532-536, (2013) (PubMed).

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." dans: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Détail du antigène

Antigène: CRP (C-Reactive Protein (CRP))

Autre désignation: C-Reactive Protein

Sujet:

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Gene Full Name: C-reactive protein

ID gène: 25419

UniProt: P48199

Pathways: Carbohydrate Homeostasis