Thrombomodulin Kit ELISA

N° du produit ABIN1706070

Détail du produit Thrombomodulin Kit ELISA

Antigène: Thrombomodulin (THBD)

Épitope: AA 17-517

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Thrombomodulin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: L17-S517

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: L17-S517

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 C-type lectin domain.

Tissue Specificity: Endothelial cells are unique in synthesizing thrombomodulin.

Plaque: Pre-coated

Protocole: mouse Thrombomodulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Thrombomodulin has been precoated onto 96-well plates. Standards(NSO, L17-S517) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thrombomodulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Thrombomodulin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Thrombomodulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Thrombomodulin standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 257, Standard deviation: 17.5, CV(%): 6.8
• Sample 2: n=16, Mean(pg/ml): 1942, Standard deviation: 101.1, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 3568, Standard deviation: 167.7, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 366, Standard deviation: 31.8, CV(%): 8.7
• Sample 2: n=24, Mean(pg/ml): 2158, Standard deviation: 153.2, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 3643, Standard deviation: 251.4, CV(%): 6.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Thrombomodulin (THBD)

Autre désignation: THBD (THBD Produits)

Synonymes: AHUS6 Kit ELISA, BDCA3 Kit ELISA, CD141 Kit ELISA, THPH12 Kit ELISA, THRM Kit ELISA, TM Kit ELISA, AI385582 Kit ELISA, THBD Kit ELISA, HAST Kit ELISA, HAST3 Kit ELISA, M-PST Kit ELISA, ST1A3/ST1A4 Kit ELISA, ST1A5 Kit ELISA, STM Kit ELISA, TL-PST Kit ELISA, thrombomodulin Kit ELISA, sulfotransferase family 1A member 3 Kit ELISA, THBD Kit ELISA, Thbd Kit ELISA, SULT1A3 Kit ELISA

Sujet:

Protein Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Background: Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74 kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).

Synonyms: Thrombomodulin,TM,Fetomodulin,CD141,Thbd,

Full Gene Name: Thrombomodulin

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 21824

UniProt: P15306