Ccl12 Kit ELISA

N° du produit ABIN1706079

Le kit ELISA Ccl12 (ABIN1706079) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris Ccl12. Ce kit ELISA a été cité dans 1 publication.

Aperçu rapide pour Ccl12 Kit ELISA (ABIN1706079)

Antigène: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Épitope: AA 23-104

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit Ccl12 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Mouse CCL12/MCP5 ELISA Kit to quantitate Mouse Ccl12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: G23-G104

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 2.3%, 4.6%, 5.6%
Inter-Assay Precision (CV%): 2.2%, 7.9%, 4.7%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour Ccl12 Kit ELISA (ABIN1706079)

Pena-Philippides, Caballero-Garrido, Lordkipanidze, Roitbak: "In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response." dans: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 287, (2017) (PubMed).

Détail du antigène

Antigène: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Autre désignation: Ccl12

Sujet:

Background: Chemokine (C-C motif) ligand 12 (CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.

Gene Full Name: C-C motif chemokine ligand 12

ID gène: 20293

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin