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SensoLyte® 390 ACE2 Activity Assay Kit

Act, FRET Fluorometric
N° du produit ABIN1882389

Aperçu rapide pour SensoLyte® 390 ACE2 Activity Assay Kit (ABIN1882389)

Antigène

Voir toutes ACE2 Kits
ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Méthode de détection

Fluorometric

Application

Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • Marque

    SensoLyte®

    Attributs du produit

    The SensoLyte® 390 ACE2 Activity Assay Kit provides a convenient assay for high throughput screening of ACE2 enzyme inhibitors and inducers using a Mc-Ala/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of Mc-Ala is quenched by Dnp. Upon cleavage into two separate fragments by ACE2, the fluorescence of Mc-Ala is recovered, and can be monitored at excitation/emission = 330/390 nm. This assay can detect the activity of subnanogram level of ACE2. Assays are performed in a convenient 96-well microplate format.
  • Commentaires

    FRET-based Assay Kit

    Restrictions

    For Research Use only
  • Conseil sur la manipulation

    Protect Components A and B from light and from moisture.

    Stock

    -20 °C

    Stockage commentaire

    Store all kit components at -20 °C. Components C and E can be stored at room temperature for convenience.
  • Liu, Liu, Miura, Tanabe, Maeda, Terayama, Turner, Zou, Komano: "Conversion of A?43 to A?40 by the successive action of angiotensin-converting enzyme 2 and angiotensin-converting enzyme." dans: Journal of neuroscience research, Vol. 92, Issue 9, pp. 1178-86, (2014) (PubMed).

    Raffai, Khang, Vanhoutte: "Angiotensin-(1-7) augments endothelium-dependent relaxations of porcine coronary arteries to bradykinin by inhibiting angiotensin-converting enzyme 1." dans: Journal of cardiovascular pharmacology, Vol. 63, Issue 5, pp. 453-60, (2014) (PubMed).

    Gao, Yallampalli, Yallampalli: "Gestational protein restriction increases angiotensin II production in rat lung." dans: Biology of reproduction, Vol. 88, Issue 3, pp. 64, (2013) (PubMed).

    Luhtala, Vaajanen, Oksala, Valjakka, Vapaatalo: "Activities of angiotensin-converting enzymes ACE1 and ACE2 and inhibition by bioactive peptides in porcine ocular tissues." dans: Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, Vol. 25, Issue 1, pp. 23-8, (2009) (PubMed).

  • Antigène

    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

    Autre désignation

    ACE2

    Sujet

    ACE2 or ACEH, angiotensin-converting enzyme 2, a homolog of angiotensin-converting enzyme, is a novel zinc metallopeptidase with specificity, tissue distribution, and function distinct from those of ACE. ACE2 may play a unique role in the renin-angiotensin system and mediate cardiovascular and renal function.
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