SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit

N° du produit ABIN1882397

Aperçu rapide pour SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit (ABIN1882397)

Antigène: Alkaline Phosphatase (ALP)

Méthode de détection: Colorimetric

Détail du produit

Marque: SensoLyte®

Attributs du produit: SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Placental alkaline phosphatase standard,

Information d'application

Commentaires:

Reporter Gene Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Keep Component A away from light.

Stock: 4 °C

Stockage commentaire: Store component B and E at -20 °C. Store other kit components at 4 °C.

Référence

Cacciatore, Leonard, Chasin: "The isolation of CHO cells with a site conferring a high and reproducible transgene amplification rate." dans: Journal of biotechnology, Vol. 164, Issue 2, pp. 346-53, (2013) (PubMed).

Détail du antigène

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase

Sujet: The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient for kinetic analysis of gene expression over a period of time.