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SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit

FRET Fluorometric
N° du produit ABIN1882431
  • Antigène Voir toutes Caspase 3 (CASP3) Kits
    Caspase 3 (CASP3)
    Méthode de détection
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Marque
    SensoLyte®
    Attributs du produit
    The SensoLyte®Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
    Top Product
    Discover our top product CASP3 Kit ELISA
  • Commentaires

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Keep Components A and B away from light
    Stock
    -20 °C
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    Fenger, Bear, Volinia, Lin, Harrington, London, Kisseberth: "Overexpression of miR-9 in mast cells is associated with invasive behavior and spontaneous metastasis." dans: BMC cancer, Vol. 14, pp. 84, (2014) (PubMed).

    Remya, Syama, Gayathri, Varma, Mohanan: "An in vitro study on the interaction of hydroxyapatite nanoparticles and bone marrow mesenchymal stem cells for assessing the toxicological behaviour." dans: Colloids and surfaces. B, Biointerfaces, Vol. 117, pp. 389-97, (2014) (PubMed).

    Son, Kim, Han, Lee, Choi, Lee, Son: "Anti-melanoma activity of Cynanchi atrati Radix is mediated by regulation of NF-kappa B activity and pro-apoptotic proteins." dans: Journal of ethnopharmacology, Vol. 153, Issue 1, pp. 250-7, (2014) (PubMed).

    Costa, Cardoso, Nóbrega, Pereira de Almeida, Bruce, Canoll, Pedroso de Lima: "MicroRNA-21 silencing enhances the cytotoxic effect of the antiangiogenic drug sunitinib in glioblastoma." dans: Human molecular genetics, Vol. 22, Issue 5, pp. 904-18, (2013) (PubMed).

    Puttabyatappa, Brogan, Vandevoort, Chaffin: "EGF-like ligands mediate progesterone's anti-apoptotic action on macaque granulosa cells." dans: Biology of reproduction, Vol. 88, Issue 1, pp. 18, (2013) (PubMed).

    Liu, Tai, Ding, Thor, Bhuta, Sun, Huang: "Arsenic trioxide synergizes with everolimus (Rad001) to induce cytotoxicity of ovarian cancer cells through increased autophagy and apoptosis." dans: Endocrine-related cancer, Vol. 19, Issue 5, pp. 711-23, (2012) (PubMed).

    Chen, Liu, Luo, Odaka, Awate, Zhou, Shen, Zheng, Lu, Huang: "Cryptotanshinone activates p38/JNK and inhibits Erk1/2 leading to caspase-independent cell death in tumor cells." dans: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 5, pp. 778-87, (2012) (PubMed).

    Jetzt, Cheng, Li, Tumer, Cohick: "A relatively low level of ribosome depurination by mutant forms of ricin toxin A chain can trigger protein synthesis inhibition, cell signaling and apoptosis in mammalian cells." dans: The international journal of biochemistry & cell biology, Vol. 44, Issue 12, pp. 2204-11, (2012) (PubMed).

    Johnson, Berger, Cortese, Lavoie: "The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 16, pp. 6283-8, (2012) (PubMed).

    Khurana, Nejadnik, Gawande, Lin, Lee, Messing, Castaneda, Derugin, Pisani, Lue, Daldrup-Link: "Intravenous ferumoxytol allows noninvasive MR imaging monitoring of macrophage migration into stem cell transplants." dans: Radiology, Vol. 264, Issue 3, pp. 803-11, (2012) (PubMed).

    Shoeneman, Ehrhart, Eickhoff, Charles, Powers, Thamm: "Expression and function of survivin in canine osteosarcoma." dans: Cancer research, Vol. 72, Issue 1, pp. 249-59, (2012) (PubMed).

    Wittenburg, Bisson, Rose, Korch, Thamm: "The histone deacetylase inhibitor valproic acid sensitizes human and canine osteosarcoma to doxorubicin." dans: Cancer chemotherapy and pharmacology, Vol. 67, Issue 1, pp. 83-92, (2011) (PubMed).

    Ansari, Kasiri, Grant, Mandal: "Fe(III)-salen and salphen complexes induce caspase activation and apoptosis in human cells." dans: Journal of biomolecular screening, Vol. 16, Issue 1, pp. 26-35, (2011) (PubMed).

    Fossey, Bear, Lin, Li, Schwartz, Li, Fuchs, Fenger, Kisseberth, London: "The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines." dans: BMC cancer, Vol. 11, pp. 112, (2011) (PubMed).

    Gorski, Huffman, Chittur, Midura, Black, Oxford, Seidah: "Inhibition of proprotein convertase SKI-1 blocks transcription of key extracellular matrix genes regulating osteoblastic mineralization." dans: The Journal of biological chemistry, Vol. 286, Issue 3, pp. 1836-49, (2011) (PubMed).

    Rajaiah, Puttabyatappa, Polumuri, Moudgil: "Interleukin-27 and interferon-gamma are involved in regulation of autoimmune arthritis." dans: The Journal of biological chemistry, Vol. 286, Issue 4, pp. 2817-25, (2011) (PubMed).

    Shin, Wang, Park, Choi, Kim, Son: "Herbal formula CGX ameliorates LPS/D-galactosamine-induced hepatitis." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 49, Issue 6, pp. 1329-34, (2011) (PubMed).

    Jing Yu, Yumin Xu, Khaoustov, Yoffe: "Identification of components of grape powder with anti-apoptotic effects." dans: Toxicology and industrial health, Vol. 27, Issue 1, pp. 19-28, (2011) (PubMed).

    Chen, Xu, Liu, Luo, Yin, Zhou, Chen, Shen, Han, Huang: "Hydrogen peroxide inhibits mTOR signaling by activation of AMPKalpha leading to apoptosis of neuronal cells." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 5, pp. 762-73, (2010) (PubMed).

  • Antigène
    Caspase 3 (CASP3)
    Autre désignation
    Caspase-3 (CASP3 Produits)
    Synonymes
    CPP32 Kit, CPP32B Kit, SCA-1 Kit, A830040C14Rik Kit, AC-3 Kit, Apopain Kit, CC3 Kit, Caspase-3 Kit, Lice Kit, Yama Kit, mldy Kit, xcpp32 Kit, casp3 Kit, zgc:100890 Kit, CASP-3 Kit, caspase-3 Kit, caspase 3 Kit, caspase 3 S homeolog Kit, caspase 3, apoptosis-related cysteine peptidase a Kit, caspase 3, apoptosis-related cysteine peptidase Kit, CASP3 Kit, Casp3 Kit, casp3.S Kit, casp3a Kit
    Sujet
    Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
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