SensoLyte® FDP Alkaline Phosphatase Assay Kit

N° du produit ABIN1882464

Détail du produit

Antigène: Fluorescein Diphosphate (FDP)

Méthode de détection: Fluorometric

Application: Enzyme Activity Assay (EAA)

Marque: SensoLyte®

Attributs du produit: SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments

Information d'application

Commentaires:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Store Component A at -20 °C. Store other kit components at 4 °C.

Référence

Zhang, Molenda, Fournelle, Murphy, Sahai: "Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells." dans: Biomaterials, Vol. 31, Issue 30, pp. 7653-65, (2010) (PubMed).

Arany, Koyota, Sugiyama: "Nerve growth factor promotes differentiation of odontoblast-like cells." dans: Journal of cellular biochemistry, Vol. 106, Issue 4, pp. 539-45, (2009) (PubMed).

Détail du antigène

Antigène: Fluorescein Diphosphate (FDP)

Autre désignation: FDP (FDP Produits)

Sujet: Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses.