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HABA Biotin Quantitation Kit

EAA Colorimetric
N° du produit ABIN1882419
  • Antigène Tous les produits Biotin
    Biotin
    Méthode de détection
    Colorimetric
    Conjugué
    Biotin
    Application
    Enzyme Activity Assay (EAA)
    Attributs du produit
    The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4'-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.
  • Commentaires

    Enzymatic Activity Assay kit

    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    Store Component A at -20 °C. Store all other components at 4 °C
  • Antigène
    Biotin
    Abstract
    Biotin Produits
    Classe de substances
    Chemical
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