SensoLyte® 520 HDAC Activity Assay Kit

N° du produit ABIN1882477

Aperçu rapide pour SensoLyte® 520 HDAC Activity Assay Kit (ABIN1882477)

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Méthode de détection: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Détail du produit

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with HDAC containing samples. Deacetylation of substrate sensitizes it, so that, in the second step mixing with the HDAC developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. The substrate included in the kit is cell-permeable, and the assay can be used to measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. This kit also can be used for high throughput screening of HDAC inhibitors using extracts or purified enzymes. The long wavelength fluorescence of HDAC 520 substrate is less interfered by the autofluorescence of cell components and test compounds.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Stock: -80 °C

Stockage commentaire: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Components D and G can be stored at room temperature for convenience.

Référence

Park, Ahn, Kim, Yoon, Kang, Lee, Moon, Jung, Chung, Kim: "A new synthetic HDAC inhibitor, MHY218, induces apoptosis or autophagy-related cell death in tamoxifen-resistant MCF-7 breast cancer cells." dans: Investigational new drugs, Vol. 30, Issue 5, pp. 1887-98, (2012) (PubMed).

Ehnert, Zhao, Pscherer, Freude, Dooley, Kolk, Stöckle, Nussler, Hube: "Transforming growth factor ?1 inhibits bone morphogenic protein (BMP)-2 and BMP-7 signaling via upregulation of Ski-related novel protein N (SnoN): possible mechanism for the failure of BMP therapy?" dans: BMC medicine, Vol. 10, pp. 101, (2012) (PubMed).

Kang, Kim, Ko: "A novel isoform of human LZIP negatively regulates the transactivation of the glucocorticoid receptor." dans: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 11, pp. 1746-57, (2009) (PubMed).

Détail du antigène

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: HDAC

Sujet: Histone deacetylase (HDAC) enzymes modulate gene expression through the deacetylation of lysine residues on histone proteins and act as transcriptional repressors of genes. Based on their role in cell cycling, apoptosis and differentiation, HDACs have been chosen as therapeutic targets for the treatment of cancer and neurodegenerative diseases.