SensoLyte® 490 HIV Protease Assay Kit

N° du produit ABIN1882480

Détail du produit

Antigène: HIV (Human Immunodeficiency Virus (HIV))

Reactivité: Human Immunodeficiency Virus (HIV)

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 490 HIV Protease Assay Kit uses an optimized FRET peptide substrate for a continuous measurement of HIV protease activities. This FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR with this substrate results in specific cleavage and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this kit offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect Components A and B from light.

Stock: -20 °C

Référence

Hallengärd, Haller, Petersson, Boberg, Maltais, Isaguliants, Wahren, Bråve: "Increased expression and immunogenicity of HIV-1 protease following inactivation of the enzymatic activity." dans: Vaccine, Vol. 29, Issue 4, pp. 839-48, (2011) (PubMed).

Frick, Ginzburg, Lam: "A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities." dans: Methods in molecular biology (Clifton, N.J.), Vol. 587, pp. 223-33, (2010) (PubMed).

Yu, Kabashima, Tang, Shibata, Kitazato, Kobayashi, Lee, Kai: "Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction." dans: Analytical biochemistry, Vol. 397, Issue 2, pp. 197-201, (2010) (PubMed).

Détail du antigène

Antigène: HIV (Human Immunodeficiency Virus (HIV))

Abstract: HIV Produits

Classe de substances: Virus, Virus

Sujet: HIV protease (PR) is identified as an important drug-screening target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics.