SensoLyte® AFC Plasmin Activity Assay Kit

N° du produit ABIN1882541

Aperçu rapide pour SensoLyte® AFC Plasmin Activity Assay Kit (ABIN1882541)

Antigène: Plasmin (Plasmin/fibrinolysin (PL/Fbn))

Méthode de détection: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Détail du produit

Marque: SensoLyte®

Attributs du produit: The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Protect Components A and B from light and moisture. Components D and F can be stored at room temperature for convenience.

Référence

Portelli, Siedlinski, Stewart, Postma, Nieuwenhuis, Vonk, Nurnberg, Altmuller, Moffatt, Wardlaw, Parker, Connolly, Koppelman, Sayers: "Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 2, pp. 923-34, (2014) (PubMed).

Park, Oh, Kim, Cho, Kim: "Identification of a specific haptoglobin C-terminal fragment in arthritic synovial fluid and its effect on interleukin-6 expression." dans: Immunology, Vol. 140, Issue 1, pp. 133-41, (2013) (PubMed).

Stewart, Nijmeh, Brightling, Sayers: "uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium." dans: Thorax, Vol. 67, Issue 6, pp. 477-87, (2012) (PubMed).

Tudpor, Laínez, Kwakernaak, Kovalevskaya, Verkaart, van Genesen, van der Kemp, Navis, Bindels, Hoenderop: "Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria." dans: Journal of the American Society of Nephrology : JASN, Vol. 23, Issue 11, pp. 1824-34, (2012) (PubMed).

Détail du antigène

Antigène: Plasmin (Plasmin/fibrinolysin (PL/Fbn))

Autre désignation: Plasmin

Sujet: Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.