SensoLyte® Green SIRT2 Assay Kit

N° du produit ABIN1882555

Aperçu rapide pour SensoLyte® Green SIRT2 Assay Kit (ABIN1882555)

Antigène: SIRT2 (Sirtuin 2 (SIRT2))

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Détail du produit

Marque: SensoLyte®

Attributs du produit: The SensoLyte® Green SIRT2 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 2 activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with SIRT2 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step, mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. This kit can be used for high throughput screening (HTS) of SIRT2 inhibitors/activators. The long wavelength fluorescence of SIRT2 substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well plate format. 100 assays (96-well plate)

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Stock: -80 °C

Stockage commentaire: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Détail du antigène

Antigène: SIRT2 (Sirtuin 2 (SIRT2))

Autre désignation: SIRT2

Sujet: Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD+ as a cofactor. Sirtuin 2 (SIRT2) is a cytoplasmic protein involved in regulation of cell cycle, DNA repair and tumorogenesis.