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SensoLyte® Green SIRT2 Assay Kit

FRET Fluorometric
N° du produit ABIN1882555
  • Antigène Voir toutes SIRT2 Kits
    SIRT2 (Sirtuin 2 (SIRT2))
    Méthode de détection
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Marque
    SensoLyte®
    Attributs du produit
    The SensoLyte® Green SIRT2 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 2 activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with SIRT2 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step, mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. This kit can be used for high throughput screening (HTS) of SIRT2 inhibitors/activators. The long wavelength fluorescence of SIRT2 substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well plate format. 100 assays (96-well plate)
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    Discover our top product SIRT2 Kit ELISA
  • Commentaires

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.
    Stock
    -80 °C
    Stockage commentaire
    Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.
  • Antigène
    SIRT2 (Sirtuin 2 (SIRT2))
    Autre désignation
    SIRT2 (SIRT2 Produits)
    Synonymes
    CG5085 Kit, D.mel2 Kit, Dmel\\CG5085 Kit, SIRT2 Kit, dSIRT2 Kit, sir2 Kit, Sirt2 Kit, BmSirt2 Kit, AtSRT2 Kit, SIRTUIN 2 Kit, T2K12.8 Kit, sirtuin 2 Kit, SIR2 Kit, SIR2L Kit, SIR2L2 Kit, 5730427M03Rik Kit, Sir2l Kit, wu:fb57d05 Kit, zgc:55966 Kit, zgc:77003 Kit, Sirtuin 2 Kit, sirtuin 2 Kit, sirtuin 2 S homeolog Kit, NAD-dependent deacetylase sirtuin 2 homolog Kit, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) Kit, Sirt2 Kit, SIRT2 Kit, sirt2.S Kit, SRT2 Kit, sirt2 Kit
    Sujet
    Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD+ as a cofactor. Sirtuin 2 (SIRT2) is a cytoplasmic protein involved in regulation of cell cycle, DNA repair and tumorogenesis.
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