CCL25 Kit ELISA

N° du produit ABIN1889341

Kit ELISA Humain CCL25, test Colorimetric pour la quantification de Humain CCL25.

Aperçu rapide pour CCL25 Kit ELISA (ABIN1889341)

Antigène: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Épitope: AA 24-150

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit CCL25 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: For quantitative detection of human CCL25 in cell culture supernates, serum and plasma(heparin, EDTA).

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Natural and recombinant human CCL25

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Ingrédients:

• 96-well plate precoated with anti- human CCL25 antibod - 1
• Lyophilized recombinant human CCL25 standar - 10ng/tubex2
• Biotinylated anti- human CCL25 antibod - 130μl(dilution 1:100)
• Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
• Sample diluent buffe - 30ml
• Antibody diluent buffe - 12ml
• ABC diluent buffe - 12ml
• TMB color developing agen - 10ml
• TMB stop solutio - 10ml

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: Q24-L150

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human CCL25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL25 has been precoated onto 96-well plates. Standards(E.coli, Q24-L150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL25 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL25 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL25 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 423, Standard deviation: 15.23, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 1094, Standard deviation: 49.23, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 2516, Standard deviation: 158.51, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 461, Standard deviation: 28.12, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 1273, Standard deviation: 85.29, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 2338, Standard deviation: 168.34, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Autre désignation: CCL25

Sujet: Chemokine(C-C motif) ligand 25(CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK(Thymus-Expressed Chemokine). The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9

ID gène: 6370

UniProt: O15444