N-Cadherin Kit ELISA

N° du produit ABIN1889345

Détail du produit N-Cadherin Kit ELISA

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Épitope: AA 160-724

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cadherin-2/N-Cadherin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: D160-A724

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: D160-A724

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse Cadherin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NSO, D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cadherin-2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Cadherin-2 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 14.5, Standard deviation: 0.609, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 31.8, Standard deviation: 1.24, CV(%): 3.9
• Sample 3: n=16, Mean(ng/ml): 41.2, Standard deviation: 2.11, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 15.5, Standard deviation: 0.899, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 34.2, Standard deviation: 1.78, CV(%): 5.2
• Sample 3: n=24, Mean(ng/ml): 42.5, Standard deviation: 2.72, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Autre désignation: CDH2 (CDH2 Produits)

Synonymes: CD325 Kit ELISA, CDHN Kit ELISA, CDw325 Kit ELISA, NCAD Kit ELISA, N-cadherin Kit ELISA, cadherin-2 Kit ELISA, cdhn Kit ELISA, ncad Kit ELISA, cdw325 Kit ELISA, CDH2 Kit ELISA, Cadherin-2 Kit ELISA, Ncad Kit ELISA, glo Kit ELISA, lyr Kit ELISA, pac Kit ELISA, wu:fb47h04 Kit ELISA, cadherin 2 Kit ELISA, cadherin 2 S homeolog Kit ELISA, cadherin 2, type 1, N-cadherin (neuronal) Kit ELISA, CDH2 Kit ELISA, cdh2.S Kit ELISA, cdh2 Kit ELISA, Cdh2 Kit ELISA

Sujet:

Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. .

Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Synonyms: Cadherin-2,Neural cadherin,N-cadherin,CD325,Cdh2,

Full Gene Name: Cadherin-2

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 12558

UniProt: P15116

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane