Periostin Kit ELISA

N° du produit ABIN1889359

Kit ELISA Souris Periostin, test Colorimetric pour la quantification de Souris Periostin et a été mentionné dans 1 publication.

Aperçu rapide pour Periostin Kit ELISA (ABIN1889359)

Antigène: Periostin (POSTN)

Épitope: AA 24-836

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.7-6000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit Periostin Kit ELISA

Seuil minimal de détection: 93.7 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Periostin/OSF2

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: N24-Q836

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: N24-Q836

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 EMI domain.

Tissue Specificity: Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .

Plaque: Pre-coated

Protocole: mouse Periostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Periostin has been precoated onto 96-well plates. Standards(NSO, N24-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Periostin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m mouse Periostin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Periostin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 783, Standard deviation: 49.33, CV(%): 6.3
• Sample 2: n=16, Mean(pg/ml): 1721, Standard deviation: 98.1, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 2910, Standard deviation: 142.6, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 867, Standard deviation: 63.3, CV(%): 7.3
• Sample 2: n=24, Mean(pg/ml): 1834, Standard deviation: 143.1, CV(%): 7.8
• Sample 3: n=24, Mean(pg/ml): 3127, Standard deviation: 172, CV(%): 5.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Periostin Kit ELISA (ABIN1889359)

Krencik, Hokanson, Narayan, Dvornik, Rooney, Rauen, Weiss, Rowitch, Ullian: "Dysregulation of astrocyte extracellular signaling in Costello syndrome." dans: Science translational medicine, Vol. 7, Issue 286, pp. 286ra66, (2015) (PubMed).

Détail du antigène

Antigène: Periostin (POSTN)

Autre désignation: POSTN

Sujet:

Protein Function: Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .

Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

Synonyms: Periostin,PN,Osteoblast-specific factor 2,OSF-2,Postn,Osf2,

Full Gene Name: Periostin

Cellular Localisation: Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.

ID gène: 50706

UniProt: Q62009