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SAA1 Kit ELISA (Serum Amyloid A1) Kit ELISA

SAA1 Reactivité: Souris AA 20-122 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
N° du produit ABIN1889362
486,73 €
Plus frais de livraison 40,00 € et TVA
96 tests
local_shipping Destination: France
Envoi sous 8 à 11 jours ouvrables
  • Antigène
    SAA1
    Épitope
    AA 20-122
    Reactivité
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56-100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAA
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: G20-Y122
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <150pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: G20-Y122
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Detected in blood plasma (at protein level). Detected in liver. .

    Plaque
    Pre-coated
    Protocole
    mouse SAA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA has been precoated onto 96-well plates. Standards(E.coli, G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAA amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse SAA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse SAA standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.4, Standard deviation: 0.06, CV(%): 4.3
    • Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.177, CV(%): 5.2
    • Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.291, CV(%): 5.1,
    • Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.081, CV(%): 5.4
    • Sample 2: n=24, Mean(ng/ml): 3.6, Standard deviation: 0.223, CV(%): 6.2
    • Sample 3: n=24, Mean(ng/ml): 6.8, Standard deviation: 0.388, CV(%): 5.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène
    SAA1
    Autre désignation
    SAA1 (SAA1 ELISA Kit Extrait)
    Synonymes
    PIG4, SAA, SAA2, TP53I4, zgc:103580, SAA1, Saa-1, Saa2, serum amyloid A1, serum amyloid A, amyloid protein A, serum amyloid A protein, serum amyloid a protein, serum amyloid A 1, serum amyloid protein A, SAA1, saa, LOC694827, Sden_2438, Mmc1_0571, Riean_0971, Saa1, LOC100009168, LOC101120204
    Sujet

    Protein Function: Major acute phase protein. .

    Background: Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

    Synonyms: Serum amyloid A-1 protein,Saa1,

    Full Gene Name: Serum amyloid A-1 protein

    Cellular Localisation: Secreted.
    ID gène
    111345
    UniProt
    P05366
    Pathways
    Toll-Like Receptors Cascades
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