CCL3 Kit ELISA

N° du produit ABIN1889383

Kit ELISA Rat CCL3, test Colorimetric pour la quantification de Rat CCL3 et a été mentionné dans 1 publication.

Aperçu rapide pour CCL3 Kit ELISA (ABIN1889383)

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Épitope: AA 24-92

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8-500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Détail du produit CCL3 Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MIP-1alpha/CCL3

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A24-A92

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A24-A92

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: rat MIP-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-1 alpha has been precoated onto 96-well plates. Standards(E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MIP-1 alpha amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat MIP-1α standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MIP-1α standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 53, Standard deviation: 2.76, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 177, Standard deviation: 11.86, CV(%): 6.7
• Sample 3: n=16, Mean(pg/ml): 293, Standard deviation: 13.2, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 65, Standard deviation: 4.1, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 192, Standard deviation: 14.6, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 317, Standard deviation: 18.4, CV(%): 5.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CCL3 Kit ELISA (ABIN1889383)

Bi, Bi, Zhong, Zhang, Jin, Ma, Luo: "Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis." dans: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 2925-2930, (2017) (PubMed).

Détail du antigène

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Autre désignation: CCL3

Sujet:

Protein Function: Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Background: Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

Synonyms: C-C motif chemokine 3,Macrophage inflammatory protein 1-alpha,MIP-1-alpha,Small-inducible cytokine A3,Ccl3,Mip1a, Scya3,

Full Gene Name: C-C motif chemokine 3

Cellular Localisation: Secreted.

ID gène: 25542

UniProt: P50229

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy