CD13 Kit ELISA

N° du produit ABIN1889386

Détail du produit CD13 Kit ELISA

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Épitope: AA 69-966

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20.000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD13/Aminopeptidase N

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: K69-S966

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: K69-S966

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-cells and thymocytes. .

Plaque: Pre-coated

Protocole: mouse CD13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD13 has been precoated onto 96-well plates. Standards(NSO, K69-S966) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD13 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD13 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 3.15, Standard deviation: 0.164, CV(%): 5.2
• Sample 2: n=16, Mean(ng/ml): 6.64, Standard deviation: 0.372, CV(%): 5.6
• Sample 3: n=16, Mean(ng/ml): 13.09, Standard deviation: 0.785, CV(%): 6.0,
• Sample 1: n=24, Mean(ng/ml): 3.36, Standard deviation: 0.218, CV(%): 6.5
• Sample 2: n=24, Mean(ng/ml): 6.97, Standard deviation: 0.474, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 14.15, Standard deviation: 1.02, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Autre désignation: ANPEP (ANPEP Produits)

Synonymes: APN Kit ELISA, CD13 Kit ELISA, GP150 Kit ELISA, LAP1 Kit ELISA, P150 Kit ELISA, PEPN Kit ELISA, A2AP Kit ELISA, AAP Kit ELISA, ALPHA-2-PI Kit ELISA, API Kit ELISA, PLI Kit ELISA, AAT1 Kit ELISA, ALT1 Kit ELISA, GPT1 Kit ELISA, Gpt1 Kit ELISA, Apn Kit ELISA, pepN Kit ELISA, SC8E4A.13 Kit ELISA, 1300007J06Rik Kit ELISA, 2310022B03Rik Kit ELISA, ALT Kit ELISA, Gpt-1 Kit ELISA, AP-M Kit ELISA, AP-N Kit ELISA, Cd13 Kit ELISA, Apm Kit ELISA, KZP Kit ELISA, Lap1 Kit ELISA, rAPN Kit ELISA, APDE Kit ELISA, p150 Kit ELISA, anpep Kit ELISA, fb64c05 Kit ELISA, wu:fb64c05 Kit ELISA, zgc:136771 Kit ELISA, alanyl aminopeptidase, membrane Kit ELISA, serpin family F member 2 Kit ELISA, glutamic--pyruvic transaminase Kit ELISA, aminopeptidase N Kit ELISA, aminopeptidase Kit ELISA, aminopeptidase n Kit ELISA, glutamic pyruvic transaminase, soluble Kit ELISA, alanyl (membrane) aminopeptidase Kit ELISA, membrane alanyl aminopeptidase Kit ELISA, alanyl (membrane) aminopeptidase b Kit ELISA, alanyl aminopeptidase, membrane L homeolog Kit ELISA, ANPEP Kit ELISA, SERPINF2 Kit ELISA, GPT Kit ELISA, Gpt Kit ELISA, Apn2 Kit ELISA, Apn1 Kit ELISA, SCO2643 Kit ELISA, pepN Kit ELISA, Shewana3_0897 Kit ELISA, Alvin_1024 Kit ELISA, Ndas_3413 Kit ELISA, Saut_1544 Kit ELISA, Fbal_1877 Kit ELISA, ML5_3424 Kit ELISA, Despr_1988 Kit ELISA, Mesop_5365 Kit ELISA, Anpep Kit ELISA, LOC101744250 Kit ELISA, anpepb Kit ELISA, anpep.L Kit ELISA

Sujet:

Protein Function: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity). Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells. .

Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

Synonyms: Aminopeptidase N,AP-N,mAPN,3.4.11.2,Alanyl aminopeptidase,Aminopeptidase M,AP-M,Membrane protein p161,Microsomal aminopeptidase,CD13,Anpep,Lap-1, Lap1,

Full Gene Name: Aminopeptidase N

Cellular Localisation: Membrane, Single-pass type II membrane protein.

ID gène: 16790

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones