CD56 Kit ELISA

N° du produit ABIN1889387

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris CD56.

Aperçu rapide pour CD56 Kit ELISA (ABIN1889387)

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Épitope: AA 20-711

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625-40.000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit CD56 Kit ELISA

Seuil minimal de détection: 625 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD56/NCAM1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: L20-T711

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: L20-T711

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 2 fibronectin type-III domains.

Plaque: Pre-coated

Protocole: mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD56 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL mouse CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD56 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.281, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 17.2, Standard deviation: 0.946, CV(%): 5.5
• Sample 3: n=16, Mean(ng/ml): 28.6, Standard deviation: 1.83, CV(%): 6.4,
• Sample 1: n=24, Mean(ng/ml): 8.3, Standard deviation: 3.984, CV(%): 4.8
• Sample 2: n=24, Mean(ng/ml): 19.5, Standard deviation: 1.326, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 32.5, Standard deviation: 2.34, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Autre désignation: NCAM1

Sujet:

Protein Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellâ€"cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Synonyms: Neural cell adhesion molecule 1,N-CAM-1,NCAM-1,CD56,Ncam1,Ncam,

Full Gene Name: Neural cell adhesion molecule 1

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

ID gène: 17967

UniProt: P13595