F11R Kit ELISA

N° du produit ABIN1889392

Kit ELISA Souris F11R, test Colorimetric pour la quantification de Souris F11R.

Aperçu rapide pour F11R Kit ELISA (ABIN1889392)

Antigène: F11R (F11 Receptor (F11R))

Épitope: AA 27-238

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit F11R Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse JAM-A/F11R

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: K27-G238

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: K27-G238

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the immunoglobulin superfamily.

Plaque: Pre-coated

Protocole: mouse JAM-A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for JAM-A has been precoated onto 96-well plates. Standards(NSO, K27-G238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for JAM-A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse JAM-A amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse JAM-A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse JAM-A standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 279, Standard deviation: 14.51, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 681, Standard deviation: 44.95, CV(%): 6.6
• Sample 3: n=16, Mean(pg/ml): 1177, Standard deviation: 51.8, CV(%): 4.4,
• Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 18.6, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 814, Standard deviation: 61.9, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 1347, Standard deviation: 79.5, CV(%): 5.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: F11R (F11 Receptor (F11R))

Autre désignation: F11R

Sujet:

Protein Function: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus (By similarity). .

Background: Junctional adhesion molecule A(JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Synonyms: Junctional adhesion molecule A,JAM-A,Junctional adhesion molecule 1,JAM-1,CD321,F11r,Jam1, Jcam, Jcam1,

Full Gene Name: Junctional adhesion molecule A

Cellular Localisation: Cell junction, tight junction . Cell membrane, Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.

ID gène: 16456

UniProt: O88792

Pathways: Cell-Cell Junction Organization