Relaxin 1 Kit ELISA

N° du produit ABIN1889393

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris Relaxin 1.

Aperçu rapide pour Relaxin 1 Kit ELISA (ABIN1889393)

Antigène: Relaxin 1 (RLN1)

Épitope: AA 23-54, AA 161-185

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78-5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit Relaxin 1 Kit ELISA

Seuil minimal de détection: 78 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Relaxin 1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: R23-R54(B)&E161-C185(A)

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: R23-R54(B)&E161-C185(A)

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli, R23-R54(B) & E161-C185(A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Relaxin 1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Relaxin 1 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 688, Standard deviation: 45.41, CV(%): 6.6
• Sample 2: n=16, Mean(pg/ml): 1402, Standard deviation: 60.3, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 2366, Standard deviation: 130, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 651, Standard deviation: 48.2, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 1583, Standard deviation: 95, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 2425, Standard deviation: 174.6, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Relaxin 1 (RLN1)

Autre désignation: RLN1

Sujet:

Protein Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Synonyms: Prorelaxin 1,Relaxin B chain,Relaxin A chain,Rln1,Rln, Rlx,

Full Gene Name: Prorelaxin 1

Cellular Localisation: Secreted.

ID gène: 19773

UniProt: P47932