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TXN Kit ELISA

TXN Reactivité: Humain AA 2-105 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
N° du produit ABIN1889406
  • Antigène Voir toutes TXN Kits ELISA
    TXN (Thioredoxin (TXN))
    Épitope
    AA 2-105
    Reactivité
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10.000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Thioredoxin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: V2-V105
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: V2-V105
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the thioredoxin family.

    Plaque
    Pre-coated
    Protocole
    human Thioredoxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Thioredoxin has been precoated onto 96-well plates. Standards(E.coli, V2-V105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thioredoxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Thioredoxin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Thioredoxin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Thioredoxin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 0.89, Standard deviation: 0.05, CV(%): 5.6
    • Sample 2: n=16, Mean(ng/ml): 3.62, Standard deviation: 0.17, CV(%): 4.7
    • Sample 3: n=16, Mean(ng/ml): 6.83, Standard deviation: 0.37, CV(%): 5.4,
    • Sample 1: n=24, Mean(ng/ml): 0.87, Standard deviation: 0.07, CV(%): 8.0
    • Sample 2: n=24, Mean(ng/ml): 3.85, Standard deviation: 0.24, CV(%): 6.2
    • Sample 3: n=24, Mean(ng/ml): 7.12, Standard deviation: 0.41, CV(%): 5.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Csősz, Lábiscsák, Kalló, Márkus, Emri, Szabó, Tar, Tőzsér, Kiss, Márton: "Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers." dans: PLoS ONE, Vol. 12, Issue 5, pp. e0177282, (2017) (PubMed).

  • Antigène Voir toutes TXN Kits ELISA
    TXN (Thioredoxin (TXN))
    Autre désignation
    TXN (TXN Produits)
    Synonymes
    TRDX Kit ELISA, TRX Kit ELISA, TRX1 Kit ELISA, ADF Kit ELISA, AW550880 Kit ELISA, Trx1 Kit ELISA, Txn Kit ELISA, trx Kit ELISA, TXN Kit ELISA, SCM1.18 Kit ELISA, trxA3 Kit ELISA, thioredoxin Kit ELISA, TR-1 Kit ELISA, Trx-1 Kit ELISA, thioredoxin Kit ELISA, thioredoxin 1 Kit ELISA, thioredoxin L homeolog Kit ELISA, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Kit ELISA, thioredoxin pseudogene Kit ELISA, cytosolic thioredoxin Trx1 Kit ELISA, TXN Kit ELISA, Txn1 Kit ELISA, txn.L Kit ELISA, txn Kit ELISA, trx Kit ELISA, SCO0885 Kit ELISA, PHG25ORF099c Kit ELISA, LOC100349046 Kit ELISA, Txn Kit ELISA, trx1 Kit ELISA
    Sujet

    Protein Function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

    Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.

    Synonyms: Thioredoxin,Trx,ATL-derived factor,ADF,Surface-associated sulphydryl protein,SASP,TXN,TRDX, TRX, TRX1,

    Full Gene Name: Thioredoxin

    Cellular Localisation: Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
    ID gène
    7295
    UniProt
    P10599
    Pathways
    Carbohydrate Homeostasis, Cell RedoxHomeostasis
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