IL18BP Kit ELISA

N° du produit ABIN1889424

Détail du produit IL18BP Kit ELISA

Antigène: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Épitope: AA 31-194

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.7-6000 pg/mL

Seuil minimal de détection: 93.7 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18BP

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: T31-G194

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: T31-G194

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Tissue Specificity: Strongly expressed in heart, lung, placenta and spleen. .

Plaque: Pre-coated

Protocole: human IL-18BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1289, Standard deviation: 61.9, CV(%): 4.8
• Sample 2: n=16, Mean(pg/ml): 3561, Standard deviation: 203, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 5320, Standard deviation: 228.76, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 999, Standard deviation: 51.95, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 2895, Standard deviation: 189, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 4672, Standard deviation: 262, CV(%): 5.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Autre désignation: IL18BP (IL18BP Produits)

Synonymes: IL18BP Kit ELISA, IL-18BP Kit ELISA, Igifbp Kit ELISA, MC54L Kit ELISA, IL18BPa Kit ELISA, interleukin 18 binding protein Kit ELISA, IL18BP Kit ELISA, Il18bp Kit ELISA

Sujet:

Protein Function: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. .

Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.

Synonyms: Interleukin-18-binding protein,IL-18BP,Tadekinig-alfa,IL18BP,

Full Gene Name: Interleukin-18-binding protein

Cellular Localisation: Secreted.

ID gène: 10068

UniProt: O95998