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Corin Kit ELISA

CORIN Reactivité: Humain AA 67-1042 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
N° du produit ABIN1889425
  • Antigène Voir toutes Corin (CORIN) Kits ELISA
    Corin (CORIN) (Corin, Serine Peptidase (CORIN))
    Épitope
    AA 67-1042
    Reactivité
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Corin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: S67-N1042
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: S67-N1042
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the peptidase S1 family.

    Tissue Specificity: Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus. Detected in blood, in plasma as well as in serum (at protein level). .

    Plaque
    Pre-coated
    Protocole
    human Corin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Corin has been precoated onto 96-well plates. Standards(NSO, S67-N1042) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Corin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Corin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Corin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Corin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 1032, Standard deviation: 36.12, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 2355, Standard deviation: 89.49, CV(%): 3.8
    • Sample 3: n=16, Mean(pg/ml): 3568, Standard deviation: 146, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 897, Standard deviation: 42.2, CV(%): 4.7
    • Sample 2: n=24, Mean(pg/ml): 2018, Standard deviation: 131.17, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 3465, Standard deviation: 249.48, CV(%): 7.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Corin (CORIN) Kits ELISA
    Corin (CORIN) (Corin, Serine Peptidase (CORIN))
    Autre désignation
    CORIN (CORIN Produits)
    Synonymes
    CORIN Kit ELISA, CG2105 Kit ELISA, Dmel\\CG2105 Kit ELISA, SP75 Kit ELISA, LOC559109 Kit ELISA, AV273130 Kit ELISA, Lrp4 Kit ELISA, ATC2 Kit ELISA, CRN Kit ELISA, PEE5 Kit ELISA, TMPRSS10 Kit ELISA, corin, serine peptidase Kit ELISA, CG2105 gene product from transcript CG2105-RC Kit ELISA, novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN) Kit ELISA, corin Kit ELISA, CORIN Kit ELISA, Corin Kit ELISA, LOC559109 Kit ELISA
    Sujet

    Protein Function: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide.

    Background: Atrial natriuretic peptide-converting enzyme, also called CRN or ATC2, is a protein that in humans is encoded by the CORIN gene. It is mapped to 4p12. This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. It has been found that Corin is the pro-ANP-converting enzyme and that Corin-mediated pro-ANP activation may play a role in regulating blood pressure. What’s more, Corin and ANP are essential for physiologic changes at the maternal-fetal interface. This protein may also function as a pro-brain-type natriuretic peptide convertase. Corin gene mutations which cause Corin to impair activity might be an underlying mechanism in hypertension.

    Synonyms: Atrial natriuretic peptide-converting enzyme,3.4.21.-,Corin,Heart-specific serine proteinase ATC2,Pro-ANP-converting enzyme,Transmembrane protease serine 10,Atrial natriuretic peptide-converting enzyme, N-terminal propeptide,Atrial natriuretic peptide-converting enzyme, activated protease fragment,Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment,Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment,Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment,CORIN,CRN, TMPRSS10,

    Full Gene Name: Atrial natriuretic peptide-converting enzyme

    Cellular Localisation: Cell membrane, Single- pass type II membrane protein . May easily detached from the endothelial cell membrane.
    ID gène
    10699
    UniProt
    Q9Y5Q5
    Pathways
    Regulation of Systemic Arterial Blood Pressure by Hormones
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