Cathepsin E Kit ELISA

N° du produit ABIN1889446

Détail du produit Cathepsin E Kit ELISA

Antigène: Cathepsin E (CTSE)

Épitope: AA 18-396

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin E

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q18-P396

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q18-P396

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B- lymphocytes. .

Plaque: Pre-coated

Protocole: human Cathepsin E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin E has been precoated onto 96-well plates. Standards(NSO, Q18-P396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin E amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin E standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.95, Standard deviation: 0.099, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 4.67, Standard deviation: 0.201, CV(%): 4.3
• Sample 3: n=16, Mean(ng/ml): 6.05, Standard deviation: 0.218, CV(%): 3.6,
• Sample 1: n=24, Mean(ng/ml): 2.34, Standard deviation: 0.149, CV(%): 6.4
• Sample 2: n=24, Mean(ng/ml): 4.83, Standard deviation: 0.28, CV(%): 5.8
• Sample 3: n=24, Mean(ng/ml): 6.58, Standard deviation: 0.349, CV(%): 5.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Cathepsin E (CTSE)

Autre désignation: CTSE (CTSE Produits)

Synonymes: CATE Kit ELISA, A430072O03Rik Kit ELISA, C920004C08Rik Kit ELISA, CE Kit ELISA, CatE Kit ELISA, CEA Kit ELISA, CEB Kit ELISA, Ctsea Kit ELISA, CTSE Kit ELISA, ce1 Kit ELISA, ce2 Kit ELISA, cate Kit ELISA, CE1 Kit ELISA, ce1-A Kit ELISA, ctse Kit ELISA, CE2 Kit ELISA, ce2-A Kit ELISA, cathepsin E Kit ELISA, cathepsin E L homeolog Kit ELISA, cathepsin E S homeolog Kit ELISA, CTSE Kit ELISA, Ctse Kit ELISA, TP03_0056 Kit ELISA, Smp_184930 Kit ELISA, ctse Kit ELISA, ctse.L Kit ELISA, ctse.S Kit ELISA

Sujet:

Protein Function: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. .

Background: Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

Synonyms: Cathepsin E,3.4.23.34,Cathepsin E form I,Cathepsin E form II,CTSE,

Full Gene Name: Cathepsin E

Cellular Localisation: Endosome . The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.

ID gène: 1510

UniProt: P14091