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CD70 Kit ELISA

CD70 Reactivité: Souris AA 45-195 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889449
  • Antigène Voir toutes CD70 Kits ELISA
    CD70 (CD70 Molecule (CD70))
    Épitope
    AA 45-195
    Reactivité
    • 4
    • 4
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD70/TNFSF7/cd27L
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: S45-P195
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: S45-P195
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Very low level of expression. Detected in splenocytes and thymocytes.

    Plaque
    Pre-coated
    Protocole
    mouse CD70 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70 has been precoated onto 96-well plates. Standards(NSO, S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD70 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD70 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD70 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 94, Standard deviation: 3.57, CV(%): 3.8
    • Sample 2: n=16, Mean(pg/ml): 511, Standard deviation: 26.06, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 1032, Standard deviation: 44.38, CV(%): 4.3,
    • Sample 1: n=24, Mean(pg/ml): 148, Standard deviation: 6.66, CV(%): 4.5
    • Sample 2: n=24, Mean(pg/ml): 525, Standard deviation: 32.55, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 1385, Standard deviation: 77.56, CV(%): 5.6
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CD70 Kits ELISA
    CD70 (CD70 Molecule (CD70))
    Autre désignation
    CD70 (CD70 Produits)
    Synonymes
    CD27LG Kit ELISA, Cd27l Kit ELISA, Tnfsf7 Kit ELISA, CD27L Kit ELISA, TNFSF7 Kit ELISA, CD70 antigen Kit ELISA, CD70 molecule Kit ELISA, Cd70 molecule Kit ELISA, Cd70 Kit ELISA, CD70 Kit ELISA
    Sujet

    Protein Function: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. .

    Background: CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24 % amino acid sequence identity in the extracellular region.

    Synonyms: CD70 antigen,CD27 ligand,CD27-L,Tumor necrosis factor ligand superfamily member 7,CD70,Cd70,Cd27l, Cd27lg, Tnfsf7,

    Full Gene Name: CD70 antigen

    Cellular Localisation: Membrane, Single-pass type II membrane protein.
    ID gène
    21948
    UniProt
    O55237
    Pathways
    Cancer Immune Checkpoints
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