CAPN10 Kit ELISA

N° du produit ABIN7540316

Ce kit ELISA Chemical CAPN10 est un kit ELISA Colorimetric conçu pour quantifier Chemical CAPN10.

Aperçu rapide pour CAPN10 Kit ELISA (ABIN7540316)

Antigène: CAPN10 (Calpain 10 (CAPN10))

Reactivité: Chemical

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 1-243 pM/mL

Application: ELISA

Type d'échantillon: Serum, Plasma, Saliva, Cell Culture Supernatant, Urine

Détail du produit CAPN10 Kit ELISA

Seuil minimal de détection: 1 pM/mL

Fonction: Quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

Analytical Method: Quantitative

Specificité: Easy - use: All reagents and buffers for cAMP test are provided.

Réactivité croisée (Details): No significant cross-reactivity of similar compounds was found

Sensibilité: 0.61 pmol/mL

Attributs du produit: cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Ingrédients: Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cAMP mAb: 12 ml
HRP-cAMP: 6 ml
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml): 1.5 ml
cAMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy

Information d'application

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole: The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not freeze the kit.

Stock: 4 °C

Stockage commentaire: The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeks at 2-8 °C.

Date de péremption: 12 months

Détail du antigène

Antigène: CAPN10 (Calpain 10 (CAPN10))

Autre désignation: cAMP / Cyclic AMP

Classe de substances: Chemical

Sujet: CAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases.

Pathways: Positive Regulation of Peptide Hormone Secretion