Cyclic GMP Kit ELISA

N° du produit ABIN2018461

Détail du produit Cyclic GMP Kit ELISA

Antigène: Cyclic GMP (cGMP)

Reactivité: Chemical

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.3-66.7 pM/mL

Seuil minimal de détection: 0.3 pM/mL

Application: ELISA

Fonction: Quantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

Type d'échantillon: Serum, Plasma, Saliva, Cell Culture Supernatant, Urine

Analytical Method: Quantitative

Specificité: Easy - use: All reagents and buffers for cGMP test are provided.

Réactivité croisée (Details): No significant cross-reactivity of similar compounds was found.

Sensibilité: 0.11 pmol/mL

Attributs du produit: cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs). The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.

Ingrédients: Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cGMP pAb: 12 ml
HRP-cGMP: 6 ml
cGMP Standards (0, 0.3, 0.8, 2.5, 7.4, 22.2, 66.7 pmol/ml): 1.5 ml
cGMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy

Information d'application

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not freeze the kit.

Stock: 4 °C

Stockage commentaire: The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C.

Date de péremption: 12 months

Détail du antigène

Antigène: Cyclic GMP (cGMP)

Autre désignation: cGMP (cGMP Produits)

Classe de substances: Chemical

Sujet: CGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs).