CCL22 Kit ELISA

N° du produit ABIN2042381

Détail du produit CCL22 Kit ELISA

Antigène: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Marque: BioAssay™

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients: 96-well plate pre-coated with anti-CCL22/MDC, Human
Lyophilized recombinant CCL22/MDC, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Human CCL22/MDC in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Autre désignation: CCL22/MDC (CCL22 Produits)

Synonymes: ABCD-1 Kit ELISA, DC/B-CK Kit ELISA, MDC Kit ELISA, SCYA22 Kit ELISA, STCP-1 Kit ELISA, DCBCK Kit ELISA, Scya22 Kit ELISA, Mdc Kit ELISA, CCL22 Kit ELISA, C-C motif chemokine ligand 22 Kit ELISA, chemokine (C-C motif) ligand 22 Kit ELISA, CCL22 Kit ELISA, Ccl22 Kit ELISA

ID gène: 6367

UniProt: O00626